Protein profile

PA5160

drug efflux transporter

Genome: NC_002516.2

Gene: pmrB PA5160 Structure source: AlphaFold UniProt O85163 UniProt G3XD81
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Amino acids 509
Annotations 6
Features 57
PDB binders 1
Druggability 0.927

Overview

Basic information about this protein and its source genome.

Accession
PA5160
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
pmrB PA5160
Status
annotated
Amino acids
509
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.927
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence Features

Domain/signature hits from InterPro and related databases.

57 records
Show feature table
Start End DB Term Name
251 269 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
106 128 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
360 386 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
472 494 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 499 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
15 499 InterPro IPR020846 Major facilitator superfamily domain
8 496 SUPERFAMILY SSF103473 MFS general substrate transporter
8 496 InterPro IPR036259 MFS transporter superfamily
34 52 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
325 335 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
199 221 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
231 253 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 22 SignalP_EUK SignalP-noTM SignalP-noTM
270 291 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
14 493 NCBIfam TIGR00711 DHA2 family efflux MFS transporter permease subunit
14 493 InterPro IPR004638 Drug resistance transporter EmrB-like
73 78 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
387 397 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
336 354 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
398 420 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
303 324 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
140 161 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
167 189 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 33 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
221 231 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
53 72 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
302 324 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
79 100 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
336 354 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
476 494 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
265 287 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
140 162 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
17 488 PANTHER PTHR42718 MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC
101 105 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
263 436 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
263 436 InterPro IPR036259 MFS transporter superfamily
364 386 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
201 220 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
50 72 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
421 475 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
111 133 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
292 302 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
79 101 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
162 166 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
19 413 Pfam PF07690 Major Facilitator Superfamily
19 413 InterPro IPR011701 Major facilitator superfamily
19 495 CDD cd17503 MFS_LmrB_MDR_like
190 200 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
167 186 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
129 139 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
232 250 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
355 359 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
16 201 Gene3D G3DSA:1.20.1720.10 Multidrug resistance protein D
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 220 FunFam G3DSA:1.20.1720.10:FF:000002 Multidrug resistance protein B
495 509 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5160
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.927
3 0.511

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

144 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
J0M
6E9O
J7QAK3 196.2 Da LogP -3.49 TPSA 138.5 1 viol. ✓ Clean C([C@H]([C@@H]([C@@H]([C@H](C(=O)O)O)O)O)O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.