Protein profile

PA5165

two-component sensor

Genome: NC_002516.2

Gene: PA5165 dctB Structure source: AlphaFold UniProt Q9HU20
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Amino acids 612
Annotations 8
Features 46
PDB binders 6
Druggability 0.555

Overview

Basic information about this protein and its source genome.

Accession
PA5165
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5165 dctB
Status
annotated
Amino acids
612
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0032892 Any process that activates or increases the frequency, rate or extent of the directed movement of organic acids into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.555
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0032892 Any process that activates or increases the frequency, rate or extent of the directed movement of organic acids into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

46 records
Show feature table
Start End DB Term Name
48 276 Pfam PF02743 Cache domain
48 276 InterPro IPR033479 Double Cache domain 1
113 190 FunFam G3DSA:3.30.450.20:FF:000127 C4-dicarboxylate transport sensor protein
354 441 FunFam G3DSA:1.10.287.130:FF:000049 C4-dicarboxylate transport sensor protein DctB
385 599 ProSiteProfiles PS50109 Histidine kinase domain profile.
385 599 InterPro IPR005467 Histidine kinase domain
378 446 SMART SM00388 HisKA_10
378 446 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
328 376 Coils Coil Coil
20 42 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
43 291 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
292 312 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
490 595 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
490 595 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
447 597 Gene3D G3DSA:3.30.565.10 -
447 597 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
437 595 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
437 595 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
49 110 Gene3D G3DSA:6.10.250.3020 -
313 612 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
366 442 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
366 442 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
21 42 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
376 442 CDD cd00082 HisKA
376 442 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
488 599 SMART SM00387 HKATPase_4
488 599 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
324 371 CDD cd22249 UDM1_RNF168_RNF169-like
291 313 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
191 282 Gene3D G3DSA:3.30.450.20 PAS domain
192 595 PANTHER PTHR43065 SENSOR HISTIDINE KINASE
4 605 PIRSF PIRSF036431 HK_DctB
4 605 InterPro IPR017055 Signal transduction histidine kinase, DctB (C4-dicarboxylate transport system regulator)
68 203 SUPERFAMILY SSF103190 Sensory domain-like
68 203 InterPro IPR029151 Periplasmic sensor-like domain superfamily
113 190 Gene3D G3DSA:3.30.450.20 PAS domain
354 441 Gene3D G3DSA:1.10.287.130 -
528 542 PRINTS PR00344 Bacterial sensor protein C-terminal signature
528 542 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
546 556 PRINTS PR00344 Bacterial sensor protein C-terminal signature
546 556 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
559 577 PRINTS PR00344 Bacterial sensor protein C-terminal signature
559 577 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
379 442 Pfam PF00512 His Kinase A (phospho-acceptor) domain
379 442 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5165
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.555
1 0.438
2 0.315

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

56 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
6AZR
Q9WZV7 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
C2E
5IDM
Q9X688 690.4 Da LogP -3.05 TPSA 349.6 3 viol. ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@H]4[C@H](O3)CO[P@@](=O…
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO
PYR
4JGO
O31671 88.1 Da LogP -0.34 TPSA 54.4 ✓ Ro5 ✓ Clean CC(=O)C(=O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.