Protein profile

PA5241

exopolyphosphatase

Genome: NC_002516.2

Gene: ppx PA5241 Structure source: AlphaFold UniProt Q9ZN70
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Amino acids 506
Annotations 14
Features 18
PDB binders 6
Druggability 0.362

Overview

Basic information about this protein and its source genome.

Accession
PA5241
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
ppx PA5241
Status
annotated
Amino acids
506
Structure source
AlphaFold
EC
3.6.1.11 2.7.4.1
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0004309 Catalysis of the reaction: polyphosphate(n) + H2O = polyphosphate(n-1) + phosphate. GO:0008976 Catalysis of the reaction: ATP + phosphate(n) = ADP + phosphate(n+1). GO:0071978 Bacterial-type flagellum-dependent cell motility in which the action of numerous flagella results in the smooth movement of a group of cells along a solid surface. Swarming motility is observed in groups of bacteria. GO:0071977 Bacterial-type flagellum-dependent cell motility that results in the smooth movement of a cell through a liquid medium. GO:0006995 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nitrogen.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.362
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 EC 12 GO

Enzyme Commission (EC)

2

Gene Ontology (GO)

12
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0004309 Catalysis of the reaction: polyphosphate(n) + H2O = polyphosphate(n-1) + phosphate.
  • GO:0008976 Catalysis of the reaction: ATP + phosphate(n) = ADP + phosphate(n+1).
  • GO:0071978 Bacterial-type flagellum-dependent cell motility in which the action of numerous flagella results in the smooth movement of a group of cells along a solid surface. Swarming motility is observed in groups of bacteria.
  • GO:0071977 Bacterial-type flagellum-dependent cell motility that results in the smooth movement of a cell through a liquid medium.
  • GO:0006995 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nitrogen.
  • GO:0016036 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of phosphate.
  • GO:0009247 The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
  • GO:0006798 The chemical reactions and pathways resulting in the breakdown of a polyphosphate, the anion or salt of polyphosphoric acid.
  • GO:0009372 The cell-cell signaling process in which single-celled organisms carry out coordinated responses by monitoring their own population density, and often also that of other microbes, by producing small, diffusible, signal molecules, detecting the concentration of these molecules, and triggering a signal transduction pathway when a certain threshold is reached. Quorum sensing can occur amongst microbial communities in the environment or within host organisms.
  • GO:0044010 A process in which planktonically growing microorganisms of the same species grow at a liquid-air interface or on a solid substrate under the flow of a liquid and produce extracellular polymers that facilitate matrix formation, resulting in a change in the organisms' growth rate and gene transcription.
  • GO:0006793 The chemical reactions and pathways involving the nonmetallic element phosphorus or compounds that contain phosphorus.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
143 317 SUPERFAMILY SSF53067 Actin-like ATPase domain
143 317 InterPro IPR043129 ATPase, nucleotide binding domain
122 301 FunFam G3DSA:3.30.420.150:FF:000001 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
6 497 PANTHER PTHR30005 EXOPOLYPHOSPHATASE
122 301 Gene3D G3DSA:3.30.420.150 Exopolyphosphatase. Domain 2
17 139 SUPERFAMILY SSF53067 Actin-like ATPase domain
17 139 InterPro IPR043129 ATPase, nucleotide binding domain
17 312 NCBIfam TIGR03706 exopolyphosphatase
17 312 InterPro IPR022371 Exopolyphosphatase
6 506 PIRSF PIRSF001267 Pyrophosphatase_GppA_Ppx
6 506 InterPro IPR030673 Pyrophosphatase, GppA/Ppx-type
316 506 Gene3D G3DSA:1.10.3210.10 Hypothetical protein af1432
18 135 FunFam G3DSA:3.30.420.40:FF:000023 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
316 506 FunFam G3DSA:1.10.3210.10:FF:000004 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
37 315 Pfam PF02541 Ppx/GppA phosphatase family
37 315 InterPro IPR003695 Ppx/GppA phosphatase
18 309 Gene3D G3DSA:3.30.420.40 -
319 505 SUPERFAMILY SSF109604 HD-domain/PDEase-like

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5241
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.227

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

56 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
G4P
6PC1
B5ZA44 603.2 Da LogP -2.22 TPSA 345.6 3 viol. ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O…
GNP
6PC2
B5ZA44 522.2 Da LogP -2.76 TPSA 301.9 3 viol. ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O…
GSP
6PC3
B5ZA44 539.2 Da LogP -2.22 TPSA 282.0 3 viol. ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O…
LMR
6PC0
B5ZA44 134.1 Da LogP -1.09 TPSA 94.8 ✓ Ro5 ✓ Clean C([C@@H](C(=O)O)O)C(=O)O
MLT
6PC0
B5ZA44 134.1 Da LogP -1.09 TPSA 94.8 ✓ Ro5 ✓ Clean C([C@H](C(=O)O)O)C(=O)O
TS6
6PC3
B5ZA44 114.1 Da LogP 0.01 TPSA 57.5 ✓ Ro5 ✓ Clean OP(=O)(O)S

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.