Protein profile

PA5259

uroporphyrinogen-III synthase

Genome: NC_002516.2

Gene: PA5259 hemD Structure source: Experimental + AlphaFold UniProt P48246
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Amino acids 251
Annotations 5
Features 12
PDB binders 0
Druggability 0.782

Overview

Basic information about this protein and its source genome.

Accession
PA5259
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5259 hemD
Status
annotated
Amino acids
251
Structure source
Experimental + AlphaFold
EC
4.2.1.75
GO
GO:0004852 Catalysis of the reaction: hydroxymethylbilane = H2O + uroporphyrinogen III. GO:0006782 The chemical reactions and pathways resulting in the formation of protoporphyrinogen IX. GO:0006780 The chemical reactions and pathways resulting in the formation of uroporphyrinogen III. GO:0033014 The chemical reactions and pathways leading to the formation of tetrapyrroles, natural pigments containing four pyrrole rings joined by one-carbon units linking position 2 of one pyrrole ring to position 5 of the next.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.782
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0004852 Catalysis of the reaction: hydroxymethylbilane = H2O + uroporphyrinogen III.
  • GO:0006782 The chemical reactions and pathways resulting in the formation of protoporphyrinogen IX.
  • GO:0006780 The chemical reactions and pathways resulting in the formation of uroporphyrinogen III.
  • GO:0033014 The chemical reactions and pathways leading to the formation of tetrapyrroles, natural pigments containing four pyrrole rings joined by one-carbon units linking position 2 of one pyrrole ring to position 5 of the next.

Sequence Features

Domain/signature hits from InterPro and related databases.

12 records
Show feature table
Start End DB Term Name
6 245 Gene3D G3DSA:3.40.50.10090 -
6 245 InterPro IPR036108 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily
35 169 Gene3D G3DSA:3.40.50.10090 -
35 169 InterPro IPR036108 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily
3 249 SUPERFAMILY SSF69618 HemD-like
3 249 InterPro IPR036108 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily
7 233 CDD cd06578 HemD
7 233 InterPro IPR003754 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase
5 250 PANTHER PTHR38042 UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC
5 250 InterPro IPR039793 Uroporphyrinogen-III synthase
17 233 Pfam PF02602 Uroporphyrinogen-III synthase HemD
17 233 InterPro IPR003754 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4ES6
X-ray 2.22 Å A
100.0% 1-251
PDBe RCSB PDBj File
Viewing
AlphaFold PA5259
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.764
3 0.201

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 6.76 0.345
2 6.28 0.315
3 1.64 0.027