Protein profile

PA5323

acetylglutamate kinase

Genome: NC_002516.2

Gene: PA5323 argB Structure source: Experimental + AlphaFold UniProt Q9HTN2
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Amino acids 301
Annotations 7
Features 22
PDB binders 20
Druggability 0.517

Overview

Basic information about this protein and its source genome.

Accession
PA5323
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5323 argB
Status
annotated
Amino acids
301
Structure source
Experimental + AlphaFold
EC
2.7.2.8
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0003991 Catalysis of the reaction: ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate-5-phosphate. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0006526 The chemical reactions and pathways resulting in the formation of arginine, 2-amino-5-(carbamimidamido)pentanoic acid. GO:0042450 OBSOLETE. The chemical reactions and pathways resulting in the formation of arginine (2-amino-5-guanidinopentanoic acid) via the intermediate compound ornithine. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.517
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0003991 Catalysis of the reaction: ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate-5-phosphate.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0006526 The chemical reactions and pathways resulting in the formation of arginine, 2-amino-5-(carbamimidamido)pentanoic acid.
  • GO:0042450 OBSOLETE. The chemical reactions and pathways resulting in the formation of arginine (2-amino-5-guanidinopentanoic acid) via the intermediate compound ornithine.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records
Show feature table
Start End DB Term Name
9 298 PANTHER PTHR23342 N-ACETYLGLUTAMATE SYNTHASE
247 267 PRINTS PR00474 Glutamate 5-kinase family signature
247 267 InterPro IPR001057 Glutamate/acetylglutamate kinase
59 73 PRINTS PR00474 Glutamate 5-kinase family signature
59 73 InterPro IPR001057 Glutamate/acetylglutamate kinase
197 224 PRINTS PR00474 Glutamate 5-kinase family signature
197 224 InterPro IPR001057 Glutamate/acetylglutamate kinase
13 297 PIRSF PIRSF000728 NAGK
13 297 InterPro IPR004662 Acetylglutamate kinase family
27 290 Hamap MF_00082 Acetylglutamate kinase [argB].
27 290 InterPro IPR037528 Acetylglutamate kinase ArgB
28 274 Pfam PF00696 Amino acid kinase family
28 274 InterPro IPR001048 Aspartate/glutamate/uridylate kinase
8 298 SUPERFAMILY SSF53633 Carbamate kinase-like
8 298 InterPro IPR036393 Acetylglutamate kinase-like superfamily
29 272 NCBIfam TIGR00761 acetylglutamate kinase
29 272 InterPro IPR004662 Acetylglutamate kinase family
6 298 FunFam G3DSA:3.40.1160.10:FF:000004 Acetylglutamate kinase
14 295 CDD cd04250 AAK_NAGK-C
14 295 InterPro IPR041727 N-Acetyl-L-glutamate kinase, cyclic
2 301 Gene3D G3DSA:3.40.1160.10 -
2 301 InterPro IPR036393 Acetylglutamate kinase-like superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 2BUF
X-ray 2.95 Å A,B,C,D,E,F,G,H,I,J,K,L
99.7% 2-301
PDBe RCSB PDBj File
Viewing
AlphaFold PA5323
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.517
8 0.295

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 14.83 0.736
2 5.38 0.255
3 0.87 0.003

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

70 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
14N
7NLT
P9WQ01 220.3 Da LogP 1.14 TPSA 43.8 ✓ Ro5 ✓ Clean CN1CCN(CC1)c2ccc(cc2)C(=O)O
5RN
7NLU
P9WQ01 159.2 Da LogP 2.37 TPSA 32.9 ✓ Ro5 ✓ Clean CC(=O)c1c[nH]c2c1cccc2
97Q
7NNB
P9WQ01 281.2 Da LogP 3.98 TPSA 33.1 ✓ Ro5 ✓ Clean c1cc2c(cc(nc2c(c1)C(F)(F)F)C(F)(F)F)O
97T
7NLQ
P9WQ01 161.2 Da LogP 2.05 TPSA 46.3 ✓ Ro5 ✓ Clean c1ccc(c(c1)c2ccno2)O
97W
7NLX
P9WQ01 213.2 Da LogP 2.96 TPSA 33.1 ✓ Ro5 ✓ Clean c1cc2c(ccnc2cc1C(F)(F)F)O
98K
7NLY
P9WQ01 152.6 Da LogP 2.22 TPSA 28.7 ✓ Ro5 ✓ Clean c1ccc2c(c1)[nH]c(n2)Cl
98Q
7NLZ
P9WQ01 215.2 Da LogP 3.20 TPSA 25.0 ✓ Ro5 ✓ Clean COc1cc2cc[nH]c2cc1C(F)(F)F
98T
7NLS
P9WQ01 278.0 Da LogP 1.78 TPSA 46.5 ✓ Ro5 ✓ Clean COC(=O)c1ccc(c(c1)I)O
98W
7NN7
P9WQ01 210.2 Da LogP 0.97 TPSA 72.8 ✓ Ro5 ✓ Clean COC(=O)c1cc(cc(c1)O)C(=O)OC
98Z
7NN8
P9WQ01 142.2 Da LogP 2.04 TPSA 39.6 ✓ Ro5 ✓ Clean c1ccc2c(c1)c(c[nH]2)C#N
9EQ
7NM0
P9WQ01 152.1 Da LogP 1.30 TPSA 57.5 ✓ Ro5 ✓ Clean CC(=O)c1c(cccc1O)O
ANP
1GS5
P0A6C8 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
DTU
2WXB
P0A6C8 154.3 Da LogP -0.43 TPSA 40.5 ✓ Ro5 ✓ Clean C([C@H]([C@H](CS)O)O)S
GGB
7NLP
P9WQ01 176.2 Da LogP -1.80 TPSA 134.5 ✓ Ro5 ✓ Clean [H]/N=C(\N)/NOCC[C@@H](C(=O)O)N
NHE
7NLO
P9WQ01 207.3 Da LogP 0.80 TPSA 66.4 ✓ Ro5 ✓ Clean C1CCC(CC1)NCCS(=O)(=O)O
NLG
2JJ4
Q6V1L5 189.2 Da LogP -0.56 TPSA 103.7 ✓ Ro5 ✓ Clean CC(=O)N[C@@H](CCC(=O)O)C(=O)O
PIY
7NLR
P9WQ01 144.2 Da LogP 2.08 TPSA 28.7 ✓ Ro5 ✓ Clean c1ccc(cc1)c2[nH]ccn2
SRT
3T7B
Q8ZA87 150.1 Da LogP -2.12 TPSA 115.1 ✓ Ro5 ✓ Clean [C@H]([C@H](C(=O)O)O)(C(=O)O)O
UOK
7NLW
P9WQ01 186.2 Da LogP 2.24 TPSA 48.8 ✓ Ro5 ✓ Clean COc1ccc2c(c1)c(c[nH]2)CC#N
X2W
2X2W
P0A6C8 269.1 Da LogP -1.01 TPSA 150.2 ✓ Ro5 ✓ Clean CC(=O)N[C@@H](CCC(=O)OP(=O)(O)O)C(=O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.