Protein profile

PA5353

glycolate oxidase iron-sulfur subunit

Genome: NC_002516.2

Gene: PA5353 glcF Structure source: AlphaFold UniProt Q9HTK5
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Amino acids 408
Annotations 7
Features 19
PDB binders 0
Druggability 0.784

Overview

Basic information about this protein and its source genome.

Accession
PA5353
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5353 glcF
Status
annotated
Amino acids
408
Structure source
AlphaFold
EC
1.1.99.14
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0019154 Catalysis of the reaction: A + glycolate = AH(2) + glyoxylate. GO:0046872 Binding to a metal ion. GO:0046296 The chemical reactions and pathways resulting in the breakdown of glycolate, the anion of hydroxyethanoic acid (glycolic acid). GO:0051536 Binding to an iron-sulfur cluster, a combination of iron and sulfur atoms.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.784
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
  • GO:0019154 Catalysis of the reaction: A + glycolate = AH(2) + glyoxylate.
  • GO:0046872 Binding to a metal ion.
  • GO:0046296 The chemical reactions and pathways resulting in the breakdown of glycolate, the anion of hydroxyethanoic acid (glycolic acid).
  • GO:0051536 Binding to an iron-sulfur cluster, a combination of iron and sulfur atoms.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
24 89 Pfam PF12838 4Fe-4S dicluster domain
24 89 InterPro IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain
303 386 Pfam PF02754 Cysteine-rich domain
303 386 InterPro IPR004017 Cysteine-rich domain
171 255 Pfam PF02754 Cysteine-rich domain
171 255 InterPro IPR004017 Cysteine-rich domain
5 408 PIRSF PIRSF000139 Glc_ox_4Fe-4S
5 408 InterPro IPR012257 Glycolate oxidase, iron-sulphur subunit
6 405 PANTHER PTHR32479 GLYCOLATE OXIDASE IRON-SULFUR SUBUNIT
2 106 Gene3D G3DSA:1.10.1060.10 -
2 106 InterPro IPR009051 Alpha-helical ferredoxin
269 289 Coils Coil Coil
75 86 ProSitePatterns PS00198 4Fe-4S ferredoxin-type iron-sulfur binding region signature.
75 86 InterPro IPR017900 4Fe-4S ferredoxin, iron-sulphur binding, conserved site
18 124 SUPERFAMILY SSF54862 4Fe-4S ferredoxins
16 44 ProSiteProfiles PS51379 4Fe-4S ferredoxin-type iron-sulfur binding domain profile.
16 44 InterPro IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain
66 89 ProSiteProfiles PS51379 4Fe-4S ferredoxin-type iron-sulfur binding domain profile.
66 89 InterPro IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5353
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.784
1 0.263