Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA5360
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: phoB PA5360
Status: annotated
Amino acids: 229
Structure source: AlphaFold

GO

GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0032993 A macromolecular complex containing both protein and DNA molecules. GO:0000156 Responds to a phosphorelay sensor to initiate a change in cell state or activity. The activity of the response regulator is regulated by transfer of a phosphate from a histidine residue in the sensor, to an aspartate residue in the response regulator. Many but not all response regulators act as transcriptional regulators to elicit a response. GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon. GO:0071978 Bacterial-type flagellum-dependent cell motility in which the action of numerous flagella results in the smooth movement of a group of cells along a solid surface. Swarming motility is observed in groups of bacteria. GO:0006817 The directed movement of phosphate ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.44

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

11 GO

Gene Ontology (GO)

11

GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO:0032993 A macromolecular complex containing both protein and DNA molecules.
GO:0000156 Responds to a phosphorelay sensor to initiate a change in cell state or activity. The activity of the response regulator is regulated by transfer of a phosphate from a histidine residue in the sensor, to an aspartate residue in the response regulator. Many but not all response regulators act as transcriptional regulators to elicit a response.
GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.
GO:0071978 Bacterial-type flagellum-dependent cell motility in which the action of numerous flagella results in the smooth movement of a group of cells along a solid surface. Swarming motility is observed in groups of bacteria.
GO:0006817 The directed movement of phosphate ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO:0080040 Any process that activates or increases the frequency, rate or extent of cellular response to phosphate starvation.
GO:0045893 Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).

Sequence Features

Domain/signature hits from InterPro and related databases.

28 records

Show feature table

Start	End	DB	Term	Name
4	117	SMART	SM00448	REC_2
4	117	InterPro	IPR001789	Signal transduction response regulator, receiver domain
125	229	FunFam	G3DSA:1.10.10.10:FF:000011	Phosphate regulon transcriptional regulator PhoB
128	226	SUPERFAMILY	SSF46894	C-terminal effector domain of the bipartite response regulators
128	226	InterPro	IPR016032	Signal transduction response regulator, C-terminal effector
130	227	ProSiteProfiles	PS51755	OmpR/PhoB-type DNA-binding domain profile.
130	227	InterPro	IPR001867	OmpR/PhoB-type DNA-binding domain
5	121	ProSiteProfiles	PS50110	Response regulatory domain profile.
5	121	InterPro	IPR001789	Signal transduction response regulator, receiver domain
138	225	CDD	cd00383	trans_reg_C
138	225	InterPro	IPR001867	OmpR/PhoB-type DNA-binding domain
3	125	SUPERFAMILY	SSF52172	CheY-like
3	125	InterPro	IPR011006	CheY-like superfamily
6	117	Pfam	PF00072	Response regulator receiver domain
6	117	InterPro	IPR001789	Signal transduction response regulator, receiver domain
5	228	PANTHER	PTHR48111	REGULATOR OF RPOS
5	228	InterPro	IPR039420	Transcriptional regulatory protein WalR-like
151	225	SMART	SM00862	Trans_reg_C_3
151	225	InterPro	IPR001867	OmpR/PhoB-type DNA-binding domain
2	85	FunFam	G3DSA:3.40.50.2300:FF:000001	DNA-binding response regulator PhoB
86	123	Gene3D	G3DSA:6.10.250.690	-
129	229	Gene3D	G3DSA:1.10.10.10	-
129	229	InterPro	IPR036388	Winged helix-like DNA-binding domain superfamily
3	226	NCBIfam	TIGR02154	phosphate regulon transcriptional regulator PhoB
3	226	InterPro	IPR011879	Signal transduction response regulator, phosphate regulon transcriptional regulatory protein PhoB
4	121	CDD	cd17618	REC_OmpR_PhoB
2	85	Gene3D	G3DSA:3.40.50.2300	-
151	225	Pfam	PF00486	Transcriptional regulatory protein, C terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA5360	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
2				0.44

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

3 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

No PDB structure with a co-crystallized ligand found for this exact protein.

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Ligand	Source crystal	UniProt (homolog)	MW · LogP · TPSA	Lipinski	PAINS	SMILES
BEF	6EB7	A0A0H2UQ68	66.0 Da LogP 0.88 TPSA 0.0	✓ Ro5	✓ Clean	`[Be-](F)(F)F`
CAC	5ED4	P71814	137.0 Da LogP -0.52 TPSA 40.1	✓ Ro5	✓ Clean	`C[As](=O)(C)[O-]`
PHS	1NXP	A0A0H2UQ68	82.0 Da LogP -0.64 TPSA 57.5	✓ Ro5	✓ Clean	`OP(=O)O`

Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL bioactivity data found for this exact protein.

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL hits found through similar proteins.

Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).

No virtual-screening candidates for this protein.

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.

PA5360