Protein profile

PA5365

phosphate uptake regulatory protein PhoU

Genome: NC_002516.2

Gene: PA5365 phoU Structure source: Experimental + AlphaFold UniProt Q51547
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Amino acids 242
Annotations 9
Features 18
PDB binders 1
Druggability 0.943

Overview

Basic information about this protein and its source genome.

Accession
PA5365
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5365 phoU
Status
annotated
Amino acids
242
Structure source
Experimental + AlphaFold
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0042803 Binding to an identical protein to form a homodimer. GO:0060326 The directed movement of a motile cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis). GO:0030643 A homeostatic process involved in the maintenance of a steady state level of phosphate ions within a cell. GO:0050922 Any process that stops, prevents, or reduces the frequency, rate or extent of the directed movement of a motile cell or organism in response to a specific chemical concentration gradient. GO:0045936 Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways involving phosphates.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.943
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0042803 Binding to an identical protein to form a homodimer.
  • GO:0060326 The directed movement of a motile cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis).
  • GO:0030643 A homeostatic process involved in the maintenance of a steady state level of phosphate ions within a cell.
  • GO:0050922 Any process that stops, prevents, or reduces the frequency, rate or extent of the directed movement of a motile cell or organism in response to a specific chemical concentration gradient.
  • GO:0045936 Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways involving phosphates.
  • GO:2000186 Any process that stops, prevents, or reduces the frequency, rate or extent of phosphate transmembrane transport.
  • GO:0050928 Any process that stops, prevents, or reduces the frequency, rate or extent of the directed movement of a motile cell or organism towards a higher concentration in a concentration gradient of a specific chemical.
  • GO:0006817 The directed movement of phosphate ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
122 234 FunFam G3DSA:1.20.58.220:FF:000001 Phosphate-specific transport system accessory protein PhoU
1 121 Gene3D G3DSA:1.20.58.220 Phosphate transport system protein phou homolog 2; domain 2
1 121 InterPro IPR038078 PhoU-like domain superfamily
2 120 FunFam G3DSA:1.20.58.220:FF:000002 Phosphate-specific transport system accessory protein PhoU
8 229 PIRSF PIRSF003107 PhoU
8 229 InterPro IPR028366 Phosphate transport system protein PhoU
122 234 Gene3D G3DSA:1.20.58.220 Phosphate transport system protein phou homolog 2; domain 2
122 234 InterPro IPR038078 PhoU-like domain superfamily
131 215 Pfam PF01895 PhoU domain
131 215 InterPro IPR026022 PhoU domain
27 113 Pfam PF01895 PhoU domain
27 113 InterPro IPR026022 PhoU domain
55 75 Coils Coil Coil
6 226 PANTHER PTHR42930 PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU
6 226 InterPro IPR028366 Phosphate transport system protein PhoU
13 234 SUPERFAMILY SSF109755 PhoU-like
13 224 NCBIfam TIGR02135 phosphate signaling complex protein PhoU
13 224 InterPro IPR028366 Phosphate transport system protein PhoU

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4Q25
X-ray 2.28 Å A,B
100.0% 1-242
PDBe RCSB PDBj File
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AlphaFold PA5365
AlphaFold — — full sequence — Loaded
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.943
4 0.295

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
PO3
1SUM
Q9X256 79.0 Da LogP -1.64 TPSA 63.2 ✓ Ro5 ✓ Clean [O-][P-](=O)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.