Protein profile

PA5366

phosphate ABC transporter ATP-binding protein

Genome: NC_002516.2

Gene: PA5366 pstB Structure source: AlphaFold UniProt Q51546
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Amino acids 277
Annotations 10
Features 19
PDB binders 7
Druggability 0.709

Overview

Basic information about this protein and its source genome.

Accession
PA5366
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5366 pstB
Status
annotated
Amino acids
277
Structure source
AlphaFold
EC
7.3.2.1
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. GO:0015415 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + phosphate(out) = ADP + phosphate + phosphate(in). GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0035435 The process in which a phosphate is transported across a membrane.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
38.298
Human E-value
9.86e-08
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.709
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MQNETASHGINFDALGRDRQSLDLASESVELEVPGLNLFYGAKQALFDVRMNIPKQRVTAFIGPSGCGKSTLLRCFNRMNDLVDGCRVEGEIRLDGHNIFAKGVDVAELRRRVGMVFQKPNPFPKSIYENVVYGLRIQGINKKRVLDEAVEWALKGAALWEEVKDRLHESALGLSGGQQQRLVIARTIAVEPEVLLLDEPCSALDPISTLKIEELIYELKSKFTIVIVTHNMQQAARVSDYTAFMYMGKLIEFGDTDTLFTNPAKKQTEDYITGRYG

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
  • GO:0015415 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + phosphate(out) = ADP + phosphate + phosphate(in).
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0035435 The process in which a phosphate is transported across a membrane.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005315 Enables the transfer of phosphate ions from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
32 277 NCBIfam TIGR00972 phosphate ABC transporter ATP-binding protein PstB
32 277 InterPro IPR005670 Phosphate import ATP-binding protein PstB-like
25 274 FunFam G3DSA:3.40.50.300:FF:000132 Phosphate import ATP-binding protein PstB
48 201 Pfam PF00005 ABC transporter
48 201 InterPro IPR003439 ABC transporter-like, ATP-binding domain
21 275 PANTHER PTHR43423 ABC TRANSPORTER I FAMILY MEMBER 17
21 275 InterPro IPR005670 Phosphate import ATP-binding protein PstB-like
28 264 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
28 264 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
174 188 ProSitePatterns PS00211 ABC transporters family signature.
174 188 InterPro IPR017871 ABC transporter-like, conserved site
26 274 Gene3D G3DSA:3.40.50.300 -
26 274 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
31 272 ProSiteProfiles PS50893 ATP-binding cassette, ABC transporter-type domain profile.
31 272 InterPro IPR003439 ABC transporter-like, ATP-binding domain
31 259 CDD cd03260 ABC_PstB_phosphate_transporter
31 259 InterPro IPR005670 Phosphate import ATP-binding protein PstB-like
55 257 SMART SM00382 AAA_5
55 257 InterPro IPR003593 AAA+ ATPase domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5366
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.709

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

57 records
Chemistry signal

Structural ligand evidence is available for this target.

Direct evidence 0 57 via homologs
Structural ligands 7 0 loaded crystals
Bioactive compounds 0 50 ZINC candidates
Drug-like & clean 22 0 PAINS alerts
Best available ligand signal
2BA PDB via homolog 658.4 Da · LogP -1.63 · TPSA 309.7
2BA PDB via homolog AGS PDB via homolog ANP PDB via homolog AT4 PDB via homolog

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
2BA
7AHE
Q9KIF7 658.4 Da LogP -1.63 TPSA 309.7 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@H]4[C@H](O3)C…
AGS
3C4J
D0VWX4 523.2 Da LogP -1.51 TPSA 262.1 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
ANP
3C41
D0VWX4 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
AT4
2OLK
D0VWX4 443.3 Da LogP -0.81 TPSA 212.4 2 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
BET
7AHH
Q9KIF7 118.2 Da LogP -0.22 TPSA 37.3 ✓ Ro5 ✓ Clean C[N+](C)(C)CC(=O)O
LMT
4MKI
Q8R7Y5 510.6 Da LogP -0.45 TPSA 178.5 3 viol. ✓ Clean CCCCCCCCCCCCO[C@H]1[C@@H]([C@H]([C@@H]([C@H](O1…
LPP
7CHA
Q9HVW1 648.9 Da LogP 10.51 TPSA 119.4 2 viol. ✓ Clean CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(=O)(O)O)OC(=O)C…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.