Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA5394
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA5394 clsA cls
Status: annotated
Amino acids: 490
Structure source: AlphaFold

EC

2.7.8.-

GO

GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0008808 Catalysis of the reaction: phosphatidylglycerol + phosphatidylglycerol = diphosphatidylglycerol (cardiolipin) + glycerol. GO:0032049 The chemical reactions and pathways resulting in the formation of cardiolipin, 1,3-bis(3-phosphatidyl)glycerol. GO:0016780 Catalysis of the transfer of a substituted phosphate group, other than diphosphate or nucleotidyl residues, from one compound (donor) to a another (acceptor). GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): N
Localization: CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.807

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

2.7.8.-

Gene Ontology (GO)

6

GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0008808 Catalysis of the reaction: phosphatidylglycerol + phosphatidylglycerol = diphosphatidylglycerol (cardiolipin) + glycerol.
GO:0032049 The chemical reactions and pathways resulting in the formation of cardiolipin, 1,3-bis(3-phosphatidyl)glycerol.
GO:0016780 Catalysis of the transfer of a substituted phosphate group, other than diphosphate or nucleotidyl residues, from one compound (donor) to a another (acceptor).
GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Sequence Features

Domain/signature hits from InterPro and related databases.

28 records

Show feature table

Start	End	DB	Term	Name
13	490	Hamap	MF_00190	Cardiolipin synthase A [clsA].
13	490	InterPro	IPR030840	Cardiolipin synthase A
1	19	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
137	292	CDD	cd09155	PLDc_PaCLS_like_1
20	42	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
47	69	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
123	301	Gene3D	G3DSA:3.30.870.10	Endonuclease Chain A
403	430	ProSiteProfiles	PS50035	Phospholipase D phosphodiesterase active site profile.
403	430	InterPro	IPR001736	Phospholipase D/Transphosphatidylase
313	462	FunFam	G3DSA:3.30.870.10:FF:000021	Cardiolipin synthase
70	490	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
41	490	PANTHER	PTHR21248	CARDIOLIPIN SYNTHASE
124	308	FunFam	G3DSA:3.30.870.10:FF:000014	Cardiolipin synthase
315	490	CDD	cd09161	PLDc_PaCLS_like_2
25	490	NCBIfam	TIGR04265	cardiolipin synthase
25	490	InterPro	IPR022924	Cardiolipin synthase
48	69	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
281	469	SUPERFAMILY	SSF56024	Phospholipase D/nuclease
403	430	SMART	SM00155	pld_4
229	256	SMART	SM00155	pld_4
229	256	InterPro	IPR001736	Phospholipase D/Transphosphatidylase
43	47	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
229	256	ProSiteProfiles	PS50035	Phospholipase D phosphodiesterase active site profile.
20	42	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
331	456	Pfam	PF13091	PLD-like domain
331	456	InterPro	IPR025202	Phospholipase D-like domain
117	319	SUPERFAMILY	SSF56024	Phospholipase D/nuclease
313	461	Gene3D	G3DSA:3.30.870.10	Endonuclease Chain A

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA5394	AlphaFold	—	—	full sequence	—	Viewing

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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
2				0.807
5				0.316
10				0.253

PA5394