Protein profile

PA5471

hypothetical protein

Genome: NC_002516.2

Gene: PA5471 Structure source: AlphaFold UniProt Q9HT99
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Amino acids 379
Annotations 9
Features 12
PDB binders 3
Druggability 0.556

Overview

Basic information about this protein and its source genome.

Accession
PA5471
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5471
Status
annotated
Amino acids
379
Structure source
AlphaFold
EC
6.5.1.8
GO
GO:0005525 Binding to GTP, guanosine triphosphate. GO:0046872 Binding to a metal ion. GO:0170057 Catalysis of the joining of an RNA with 3'-phosphate or 2',3'-cyclic-phosphate ends to an RNA with 5'-hydroxy ends according to either (i) a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP; or (ii) a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP + H+. GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms. GO:0042245 Any process that results in the repair of damaged RNA. GO:0006388 Splicing of tRNA substrates via recognition of the folded RNA structure that brings the 5' and 3' splice sites into proximity and cleavage of the RNA at both the 3' and 5' splice sites by an endonucleolytic mechanism, followed by ligation of the exons.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
27.64
Human E-value
5.14e-27
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.556
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005525 Binding to GTP, guanosine triphosphate.
  • GO:0046872 Binding to a metal ion.
  • GO:0170057 Catalysis of the joining of an RNA with 3'-phosphate or 2',3'-cyclic-phosphate ends to an RNA with 5'-hydroxy ends according to either (i) a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP; or (ii) a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP + H+.
  • GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
  • GO:0042245 Any process that results in the repair of damaged RNA.
  • GO:0006388 Splicing of tRNA substrates via recognition of the folded RNA structure that brings the 5' and 3' splice sites into proximity and cleavage of the RNA at both the 3' and 5' splice sites by an endonucleolytic mechanism, followed by ligation of the exons.
  • GO:0006396 Any process involved in the conversion of one or more primary RNA transcripts into one or more mature RNA molecules.
  • GO:0008452 Catalysis of the formation of a phosphodiester bond between a hydroxyl group at the end of one RNA chain and the phosphate group at the end of another.

Sequence Features

Domain/signature hits from InterPro and related databases.

12 records
Show feature table
Start End DB Term Name
11 372 Gene3D G3DSA:3.90.1860.10 -
11 372 InterPro IPR036025 tRNA-splicing ligase RtcB-like superfamily
29 97 Pfam PF01139 tRNA-splicing ligase RtcB
29 97 InterPro IPR001233 RNA-splicing ligase, RtcB
12 367 NCBIfam TIGR03073 RNA ligase RtcB family protein, release factor H-associated
12 367 InterPro IPR017510 Release factor H-coupled RctB family protein
8 372 SUPERFAMILY SSF103365 Hypothetical protein PH1602
8 372 InterPro IPR036025 tRNA-splicing ligase RtcB-like superfamily
125 372 Pfam PF01139 tRNA-splicing ligase RtcB
125 372 InterPro IPR001233 RNA-splicing ligase, RtcB
21 98 PANTHER PTHR11118 UNCHARACTERIZED
21 98 InterPro IPR001233 RNA-splicing ligase, RtcB

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5471
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.556
4 0.362

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
5GP
4DWQ
O59245 363.2 Da LogP -2.57 TPSA 206.0 1 viol. ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O…
MLI
4DWQ
O59245 102.0 Da LogP -3.12 TPSA 80.3 ✓ Ro5 ✓ Clean C(C(=O)[O-])C(=O)[O-]
POP
4DWQ
O59245 176.0 Da LogP -2.08 TPSA 129.9 ✓ Ro5 ✓ Clean O[P@@](=O)([O-])O[P@@](=O)(O)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.