Protein profile

PA5489

thiol:disulfide interchange protein DsbA

Genome: NC_002516.2

Gene: dsbA PA5489 Structure source: Experimental + AlphaFold UniProt P0C2B2
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Amino acids 211
Annotations 5
Features 21
PDB binders 27
Druggability 0.808

Overview

Basic information about this protein and its source genome.

Accession
PA5489
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
dsbA PA5489
Status
annotated
Amino acids
211
Structure source
Experimental + AlphaFold
GO
GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall. GO:0003756 Catalysis of the rearrangement of both intrachain and interchain disulfide bonds in proteins. GO:0015035 Catalysis of the reaction: a protein with reduced sulfide groups = a protein with oxidized disulfide bonds. GO:0071236 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms. GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Periplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.808
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
  • GO:0003756 Catalysis of the rearrangement of both intrachain and interchain disulfide bonds in proteins.
  • GO:0015035 Catalysis of the reaction: a protein with reduced sulfide groups = a protein with oxidized disulfide bonds.
  • GO:0071236 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
  • GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
1 22 Phobius SIGNAL_PEPTIDE Signal peptide region
25 203 SUPERFAMILY SSF52833 Thioredoxin-like
25 203 InterPro IPR036249 Thioredoxin-like superfamily
48 66 ProSitePatterns PS00194 Thioredoxin family active site.
48 66 InterPro IPR017937 Thioredoxin, conserved site
23 211 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 22 SignalP_EUK SignalP-noTM SignalP-noTM
27 207 CDD cd03019 DsbA_DsbA
27 207 InterPro IPR023205 Thiol:disulphide interchange protein DsbA/DsbL
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
16 22 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 210 PANTHER PTHR35891 THIOL:DISULFIDE INTERCHANGE PROTEIN DSBA
96 182 Pfam PF01323 DSBA-like thioredoxin domain
96 182 InterPro IPR001853 DSBA-like thioredoxin domain
1 22 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
20 211 Gene3D G3DSA:3.40.30.10 Glutaredoxin
1 209 PIRSF PIRSF001488 Tdi_protein
1 209 InterPro IPR023205 Thiol:disulphide interchange protein DsbA/DsbL
16 203 ProSiteProfiles PS51352 Thioredoxin domain profile.
16 203 InterPro IPR013766 Thioredoxin domain
4 15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

5 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4ZL8
X-ray 1.40 Å A
90.0% 22-211
PDBe RCSB PDBj File
Viewing
PDB 5DCH
X-ray 1.45 Å A
90.0% 22-211
PDBe RCSB PDBj File
Loaded
PDB 4ZL9
X-ray 1.70 Å A
90.0% 22-211
PDBe RCSB PDBj File
Loaded
PDB 4ZL7
X-ray 1.92 Å A
90.0% 22-211
PDBe RCSB PDBj File
Loaded
PDB 3H93
X-ray 1.50 Å A
89.6% 23-211
PDBe RCSB PDBj File
Loaded
AlphaFold PA5489
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.808

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 5.94 0.292
2 4.99 0.226
3 2.57 0.073

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

88 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
1YO
5DCH 1.45 Å Open crystal
220.3 Da LogP 1.59 TPSA 56.7 ✓ Ro5 ✓ Clean Cc1ccccc1CSc2nncn2N

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.