Protein profile

PA5512

sensor histidine kinase MifS

Genome: NC_002516.2

Gene: PA5512 mifS Structure source: AlphaFold UniProt Q9HT61
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Amino acids 588
Annotations 7
Features 45
PDB binders 5
Druggability 0.897

Overview

Basic information about this protein and its source genome.

Accession
PA5512
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA5512 mifS
Status
annotated
Amino acids
588
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.897
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

45 records
Show feature table
Start End DB Term Name
432 585 Gene3D G3DSA:3.30.565.10 -
432 585 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
299 319 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
103 178 Gene3D G3DSA:3.30.450.20 PAS domain
315 586 PANTHER PTHR43065 SENSOR HISTIDINE KINASE
542 560 PRINTS PR00344 Bacterial sensor protein C-terminal signature
542 560 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
565 578 PRINTS PR00344 Bacterial sensor protein C-terminal signature
565 578 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
511 525 PRINTS PR00344 Bacterial sensor protein C-terminal signature
511 525 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
529 539 PRINTS PR00344 Bacterial sensor protein C-terminal signature
529 539 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
433 579 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
433 579 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
1 8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
9 22 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
477 577 CDD cd16976 HATPase_HupT_MifS-like
362 430 SMART SM00388 HisKA_10
362 430 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
338 425 FunFam G3DSA:1.10.287.130:FF:000049 C4-dicarboxylate transport sensor protein DctB
47 180 CDD cd12914 PDC1_DGC_like
472 579 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
472 579 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
23 31 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
40 100 Gene3D G3DSA:6.10.250.3020 -
108 193 SUPERFAMILY SSF103190 Sensory domain-like
108 193 InterPro IPR029151 Periplasmic sensor-like domain superfamily
472 581 SMART SM00387 HKATPase_4
472 581 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
358 431 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
358 431 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
360 426 CDD cd00082 HisKA
360 426 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
1 31 Phobius SIGNAL_PEPTIDE Signal peptide region
1 588 PIRSF PIRSF036431 HK_DctB
1 588 InterPro IPR017055 Signal transduction histidine kinase, DctB (C4-dicarboxylate transport system regulator)
369 581 ProSiteProfiles PS50109 Histidine kinase domain profile.
369 581 InterPro IPR005467 Histidine kinase domain
340 425 Gene3D G3DSA:1.10.287.130 -
32 298 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 23 SignalP_EUK SignalP-noTM SignalP-noTM
179 290 Gene3D G3DSA:3.30.450.20 PAS domain
362 430 Pfam PF00512 His Kinase A (phospho-acceptor) domain
320 588 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5512
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.466
4 0.361
3 0.279

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

55 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
6QRJ
Q9ABT2 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
C2E
5IDM
Q9X688 690.4 Da LogP -3.05 TPSA 349.6 3 viol. ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@H]4[C@H](O3)CO[P@@](=O…
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.