Promising target candidate with multiple supporting evidence streams.
Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.
Main supporting evidence
Risks to review
Evidence coverage
Terms and data sources used on this page
PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.
AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.
ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.
pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.
FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.
Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.
PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.
ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.
ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.
LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.
Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.
DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.
Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.
EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.
KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.
Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.
Prioritization evidence
Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.
Off-target risk
- Human off-target
- No hit
- Gut microbiome similarity
- 2.9% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.
Essentiality
- Essential (DEG)
- Y
- DEG identity (%)
- 90.616 Higher values support similarity to known essential genes.
- DEG E-value
- 0.0 Smaller values mean stronger essential-gene similarity.
Structure confidence
- ColabFold pLDDT
- 92.64 0-100 confidence; >70 supports local structural interpretation.
Binding-site evidence
AlphaFold DB / UniProt modelP2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.
Sequence
Primary amino-acid sequence viewer.
MNTFFKITALAGLLAIAGHAFAVDDITRADQIPVLKEEPQHATVSERVTSRFTRSHYRQFDLDNAFSAKIFDRYLNLLDYSHNVLLASDVAKFAAKKDQIGDELRSGKLDVFYDLYNLGQQRRFERYQYALKVLERPMDFTGNDNFNLDRSKAPWPKDEAELNKLWDAKVKFDQLSLKLAGKDDKEIRDTLTRRYKFAIRRLAQTNSEDVFSLAMTSFAREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSLVAGGPAAKSKAISVGDRIVGVGQTGKSMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGAKTRIVTLTRERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSIIIDLRSNGGGALTEAVSLSGLFIPSGPVVQVRDNNGKVREDSDNDGVVYYKGPLVVLVDRFSASASEIFAAAMQDYGRALIVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTIQKFYRINGGSTQRKGVTPDIMMPTGNEDRETGEQYEDNALPWDSINAATYVKSGDLTPFGPELLKRHDERIAQDPEFQYIMKDIARYNAMKDKRNIVSLNYAQREKENEEDDAIRLTRINDRLKREGKPPLKKLDDLPKDYQEPDPYLDETVHIAVDLAHLEKARPAVEPPASK
Functional annotations
Enzyme classification and Gene Ontology terms linked to this protein.
Subcellular localization
- Localization
- CytoplasmicMembrane
Enzyme Commission (EC)
1Gene Ontology (GO)
7- GO:0008236 Catalysis of the hydrolysis of peptide bonds in a polypeptide chain by a catalytic mechanism that involves a catalytic triad consisting of a serine nucleophile that is activated by a proton relay involving an acidic residue (e.g. aspartate or glutamate) and a basic residue (usually histidine).
- GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
- GO:0005515 Binding to a protein.
- GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
- GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
- GO:0004252 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a catalytic mechanism that involves a catalytic triad consisting of a serine nucleophile that is activated by a proton relay involving an acidic residue (e.g. aspartate or glutamate) and a basic residue (usually histidine).
- GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
Sequence domains and features
Domain and signature matches imported from InterPro and related databases.
Show feature table
| Start | End | DB | Term | Name |
|---|---|---|---|---|
| 23 | 682 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. |
| 194 | 551 | NCBIfam | TIGR00225 | C-terminal processing peptidase |
| 194 | 551 | InterPro | IPR004447 | C-terminal-processing peptidase S41A |
| 1 | 22 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM |
| 219 | 343 | SUPERFAMILY | SSF50156 | PDZ domain-like |
| 219 | 343 | InterPro | IPR036034 | PDZ superfamily |
| 246 | 340 | FunFam | G3DSA:2.30.42.10:FF:000083 | Tail-specific protease |
| 1 | 22 | Phobius | SIGNAL_PEPTIDE | Signal peptide region |
| 633 | 654 | MobiDBLite | mobidb-lite | consensus disorder prediction |
| 7 | 17 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. |
| 238 | 308 | ProSiteProfiles | PS50106 | PDZ domain profile. |
| 238 | 308 | InterPro | IPR001478 | PDZ domain |
| 194 | 538 | SUPERFAMILY | SSF52096 | ClpP/crotonase |
| 194 | 538 | InterPro | IPR029045 | ClpP/crotonase-like domain superfamily |
| 614 | 634 | Coils | Coil | Coil |
| 240 | 320 | Pfam | PF00595 | PDZ domain |
| 240 | 320 | InterPro | IPR001478 | PDZ domain |
| 1 | 22 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM |
| 341 | 528 | Gene3D | G3DSA:3.90.226.10 | - |
| 152 | 556 | PANTHER | PTHR32060 | TAIL-SPECIFIC PROTEASE |
| 49 | 232 | Pfam | PF17804 | Tail specific protease N-terminal domain |
| 49 | 232 | InterPro | IPR040573 | Tail specific protease, N-terminal domain |
| 536 | 667 | Pfam | PF11818 | C-terminal domain of tail specific protease (DUF3340) |
| 536 | 667 | InterPro | IPR020992 | Tail specific protease, C-terminal |
| 356 | 531 | CDD | cd07560 | Peptidase_S41_CPP |
| 356 | 531 | InterPro | IPR004447 | C-terminal-processing peptidase S41A |
| 341 | 493 | FunFam | G3DSA:3.90.226.10:FF:000015 | Periplasmic tail-specific protease |
| 181 | 506 | Gene3D | G3DSA:3.30.750.44 | - |
| 326 | 531 | SMART | SM00245 | tsp_4 |
| 326 | 531 | InterPro | IPR005151 | Tail specific protease |
| 246 | 323 | SMART | SM00228 | pdz_new |
| 246 | 323 | InterPro | IPR001478 | PDZ domain |
| 356 | 528 | Pfam | PF03572 | Peptidase family S41 |
| 356 | 528 | InterPro | IPR005151 | Tail specific protease |
| 18 | 22 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. |
| 1 | 6 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. |
| 245 | 337 | CDD | cd00988 | PDZ_CTP_protease |
| 246 | 340 | Gene3D | G3DSA:2.30.42.10 | - |
| 246 | 340 | InterPro | IPR036034 | PDZ superfamily |
3D structure
Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.
How colors and pocket overlays are used
Pocket details Inspect a specific pocket, or open the full viewer
- Method
- -
- Score
- -
- Visible layer
- -
- Residues
- -
- Pocket properties
- -
Selecting a pocket opens its details and centers the viewer without clearing other active layers. Use Focus this pocket when you want to hide the rest; use Surface for the wider residue environment.
Binding pockets · P2Rank
Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2
Binding pockets · P2Rank
Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2
Binding pockets · FPocket
Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4
All structural evidence
Structural evidence
0 + 2Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.
| Entry | Method | Resolution | Chain | Coverage | Links | Status |
|---|---|---|---|---|---|---|
|
AlphaFold DB
AF_A0A0H3GQ33
|
AlphaFold DB | — | — | full sequence | — | Viewing |
|
ColabFold
VK055_0103
|
ColabFold | — | — | full sequence | — | Loaded |
Ligand evidence
Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.
Structural ligand evidence is available for this target.
Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in Target, use Open crystal to inspect it in the structure viewer.
No PDB structure with a co-crystallized ligand found for this exact protein.
Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.
Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL bioactivity data found for this exact protein.
Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL hits found through similar proteins.
Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).
No virtual-screening candidates for this protein.
PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.
Cross-references
External database identifiers for this protein, its structures, ligands, and metabolic reactions.