Protein profile

VK055_0104

heat shock, integral membrane protein

Genome: KpATCC43816

Gene: htpX AIK78732.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3H016
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Amino acids 294
Annotations 5
Features 23
PDB binders 0
Druggability 0.678

Overview

Basic information about this protein and its source genome.

Accession
VK055_0104
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
htpX AIK78732.1
Status
annotated
Amino acids
294
Structure source
AlphaFold + ColabFold
EC
3.4.24.-
GO
GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0008270 Binding to a zinc ion (Zn).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
50.0
DEG E-value
3.55e-78
Localization
CytoplasmicMembrane
ColabFold pLDDT
83.71

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.678
Structure A0A0H3H016
Pocket Pocket 15
P2Rank 0.829
Structure A0A0H3H016
Pocket Pocket 1
ColabFold model
FPocket 0.741 · Pocket 16
P2Rank 0.767 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 188 / 4744 genomes with a hit
Normalized 0.04

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
  • GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0008270 Binding to a zinc ion (Zn).

Sequence Features

Domain/signature hits from InterPro and related databases.

23 records
Show feature table
Start End DB Term Name
64 157 FunFam G3DSA:3.30.2010.10:FF:000001 Protease HtpX
219 294 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
64 157 Gene3D G3DSA:3.30.2010.10 Metalloproteases ("zincins"), catalytic domain
2 290 PANTHER PTHR43221 PROTEASE HTPX
1 23 Phobius SIGNAL_PEPTIDE Signal peptide region
32 51 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
154 174 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
19 23 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
43 288 CDD cd07335 M48B_HtpX_like
158 180 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
195 217 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
74 289 Pfam PF01435 Peptidase family M48
74 289 InterPro IPR001915 Peptidase M48
1 290 Hamap MF_00188 Protease HtpX [htpX].
1 290 InterPro IPR022919 Peptidase M48, protease HtpX, putative
33 53 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
4 18 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
175 193 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
54 153 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
24 32 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
194 218 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3H016
AlphaFold — — full sequence — Viewing
ColabFold VK055_0104
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
15 0.678
1 0.027
14 0.0
17 0.0

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.57 0.829
2 1.96 0.034
3 1.37 0.014