Target candidate with partial support; inspect missing evidence before prioritizing.
Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.
Main supporting evidence
Risks to review
Terms and data sources used on this page
PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.
AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.
ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.
pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.
FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.
Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.
PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.
ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.
ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.
LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.
Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.
DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.
Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.
EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.
KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.
Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.
Prioritization evidence
Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.
Off-target risk
- Human off-target
- No hit
- Gut microbiome similarity
- 1.5% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.
Essentiality
- Essential (DEG)
- Y
- DEG identity (%)
- 67.355 Higher values support similarity to known essential genes.
- DEG E-value
- 2.9200000000000003e-89 Smaller values mean stronger essential-gene similarity.
Structure confidence
- ColabFold pLDDT
- 75.42 0-100 confidence; >70 supports local structural interpretation.
Binding-site evidence
AlphaFold DB / UniProt modelP2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.
Sequence
Primary amino-acid sequence viewer.
MTLDLPRRFPWPTLLSVAIHGAVVAGLLYTSVHQVIEQPSPTQPIEITMVAPADLEPPPAAQPVVEPVVEPEPESEPEVVPEPPKEAPVVIHKPEPKPKPKPKPKPKPEKKVEQPKREVKPAAEPRPASPFENNNTAPARTAPSTSTAAAKPTVTAPSGPRAISRVQPSYPARAQALRIEGTVRVKFDVSPDGRIDNLQILSAQPANMFEREVKSAMRRWRYEQGRPGTGVTMTIKFRLNGVEIN
Functional annotations
Enzyme classification and Gene Ontology terms linked to this protein.
Subcellular localization
- Localization
- Cytoplasmic
Gene Ontology (GO)
6- GO:0015891 The directed movement of siderophores, low molecular weight Fe(III)-chelating substances, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
- GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
- GO:0031992 The biological transducer activity that accepts energy and converts it to another form, often by transfer to another molecule within the cell.
- GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
- GO:0098797 Any protein complex that is part of the plasma membrane.
- GO:0015031 The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
Sequence domains and features
Domain and signature matches imported from InterPro and related databases.
Show feature table
| Start | End | DB | Term | Name |
|---|---|---|---|---|
| 33 | 245 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. |
| 12 | 32 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. |
| 167 | 237 | Pfam | PF03544 | Gram-negative bacterial TonB protein C-terminal |
| 167 | 237 | InterPro | IPR037682 | TonB, C-terminal |
| 160 | 227 | SUPERFAMILY | SSF74653 | TolA/TonB C-terminal domain |
| 168 | 240 | NCBIfam | TIGR01352 | TonB family C-terminal domain |
| 168 | 240 | InterPro | IPR006260 | TonB/TolA, C-terminal |
| 129 | 153 | MobiDBLite | mobidb-lite | consensus disorder prediction |
| 33 | 164 | Pfam | PF16031 | TonB polyproline region |
| 49 | 167 | MobiDBLite | mobidb-lite | consensus disorder prediction |
| 158 | 244 | Gene3D | G3DSA:3.30.2420.10 | TonB |
| 106 | 123 | MobiDBLite | mobidb-lite | consensus disorder prediction |
| 162 | 183 | PRINTS | PR01374 | Gram-negative bacterial tonB protein signature |
| 162 | 183 | InterPro | IPR003538 | Gram-negative bacterial TonB protein |
| 97 | 107 | PRINTS | PR01374 | Gram-negative bacterial tonB protein signature |
| 97 | 107 | InterPro | IPR003538 | Gram-negative bacterial TonB protein |
| 202 | 219 | PRINTS | PR01374 | Gram-negative bacterial tonB protein signature |
| 202 | 219 | InterPro | IPR003538 | Gram-negative bacterial TonB protein |
| 183 | 201 | PRINTS | PR01374 | Gram-negative bacterial tonB protein signature |
| 183 | 201 | InterPro | IPR003538 | Gram-negative bacterial TonB protein |
| 1 | 11 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. |
| 8 | 240 | PANTHER | PTHR33446 | PROTEIN TONB-RELATED |
3D structure
Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.
How colors and pocket overlays are used
Pocket details Inspect a specific pocket, or open the full viewer
- Method
- -
- Score
- -
- Visible layer
- -
- Residues
- -
- Pocket properties
- -
Selecting a pocket opens its details and centers the viewer without clearing other active layers. Use Focus this pocket when you want to hide the rest; use Surface for the wider residue environment.
Binding pockets · P2Rank
Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2
Binding pockets · FPocket
Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4
All structural evidence
Structural evidence
0 + 2Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.
| Entry | Method | Resolution | Chain | Coverage | Links | Status |
|---|---|---|---|---|---|---|
|
AlphaFold DB
AF_A0A0H3GUY2
|
AlphaFold DB | — | — | full sequence | — | Viewing |
|
ColabFold
VK055_0270
|
ColabFold | — | — | full sequence | — | Loaded |
Cross-references
External database identifiers for this protein, its structures, ligands, and metabolic reactions.