Protein profile

VK055_0385

CRISPR-associated endonuclease Cas1

Genome: KpATCC43816

Gene: cas1 AIK79011.1 Structure source: AlphaFold + ColabFold UniProt A0A2X1RJX7
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Amino acids 291
Annotations 9
Features 15
PDB binders 0
Druggability 0.303

Overview

Basic information about this protein and its source genome.

Accession
VK055_0385
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
cas1 AIK79011.1
Status
annotated
Amino acids
291
Structure source
AlphaFold + ColabFold
EC
3.1.-.-
GO
GO:0051607 Reactions triggered in response to the presence of a virus that act to protect the cell or organism. GO:0003676 Binding to a nucleic acid. GO:0043571 Any process involved in sustaining CRISPR repeat clusters, including capture of new spacer elements, expansion or contraction of clusters, propagation of the leader sequence and repeat clusters within a genome, transfer of repeat clusters and CRISPR-associated (cas) genes to new genomes, transcription of the CRISPR repeat arrays into RNA and processing, and interaction of CRISPR/cas loci with the host genome. CRISPR (clustered regularly interspaced short palindromic repeat) elements are a family of sequence elements containing multiple direct repeats of 24-48 bp with weak dyad symmetry which are separated by regularly sized nonrepetitive spacer sequences. GO:0004520 Catalysis of the cleavage of ester linkages within deoxyribonucleic acid by creating internal breaks. GO:0046872 Binding to a metal ion. GO:0004519 Catalysis of the cleavage of ester linkages within nucleic acids by creating internal breaks.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
89.25

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.303
Structure A0A2X1RJX7
Pocket Pocket 4
P2Rank 0.335
Structure A0A2X1RJX7
Pocket Pocket 1
ColabFold model
FPocket 0.509 · Pocket 18
P2Rank 0.624 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 16 / 4744 genomes with a hit
Normalized 0.003

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0051607 Reactions triggered in response to the presence of a virus that act to protect the cell or organism.
  • GO:0003676 Binding to a nucleic acid.
  • GO:0043571 Any process involved in sustaining CRISPR repeat clusters, including capture of new spacer elements, expansion or contraction of clusters, propagation of the leader sequence and repeat clusters within a genome, transfer of repeat clusters and CRISPR-associated (cas) genes to new genomes, transcription of the CRISPR repeat arrays into RNA and processing, and interaction of CRISPR/cas loci with the host genome. CRISPR (clustered regularly interspaced short palindromic repeat) elements are a family of sequence elements containing multiple direct repeats of 24-48 bp with weak dyad symmetry which are separated by regularly sized nonrepetitive spacer sequences.
  • GO:0004520 Catalysis of the cleavage of ester linkages within deoxyribonucleic acid by creating internal breaks.
  • GO:0046872 Binding to a metal ion.
  • GO:0004519 Catalysis of the cleavage of ester linkages within nucleic acids by creating internal breaks.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
13 291 Hamap MF_01470 CRISPR-associated endonuclease Cas1 [cas1].
13 291 InterPro IPR002729 CRISPR-associated protein Cas1
19 281 CDD cd09719 Cas1_I-E
19 281 InterPro IPR033641 CRISPR-associated protein Cas1, type I-E
103 289 Gene3D G3DSA:1.20.120.920 -
103 289 InterPro IPR042206 CRISPR-associated endonuclease Cas1, C-terminal domain
140 245 Pfam PF01867 CRISPR associated protein Cas1
140 245 InterPro IPR002729 CRISPR-associated protein Cas1
139 279 NCBIfam TIGR00287 CRISPR-associated endonuclease Cas1
139 279 InterPro IPR002729 CRISPR-associated protein Cas1
140 262 PANTHER PTHR34353 CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1
12 102 Gene3D G3DSA:3.100.10.20 -
12 102 InterPro IPR042211 CRISPR-associated endonuclease Cas1, N-terminal domain
15 281 NCBIfam TIGR03638 type I-E CRISPR-associated endonuclease Cas1e
15 281 InterPro IPR019851 CRISPR-associated protein Cas1, ECOLI subtype

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A2X1RJX7
AlphaFold — — full sequence — Viewing
ColabFold VK055_0385
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.303

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 4.21 0.171
2 3.95 0.156
3 1.75 0.032
4 0.68 0.001