Genome KpATCC43816

Protein target profile

bestrophin, RFP-TM, chloride channel family protein

Accession: VK055_0657

Gene: AIK79280.1 3D evidence: Experimental + ColabFold model Metabolism Not in network UniProt W9BH30
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Length 305
Pocket druggability (P2Rank) 0.128
Functional annotation 0 EC 3 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Experimental structure available At least one PDB experimental structure is loaded, not just a predicted model - stronger structural coverage than AlphaFold/ColabFold alone. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.97. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 74 of 4744 screened genomes (1.6%) have a significant similarity hit. This target also has a predicted binding pocket (FPocket 0.97) — check the ligand evidence before deprioritizing on off-target risk alone. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.13.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
1.6% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
94.57 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

PDB experimental structure

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.128
Structure 6IVN
Pocket Pocket 1
Druggability (FPocket) 0.975
Structure 6IVM
Pocket Pocket 1
ColabFold model
P2Rank 0.054 · Pocket 1
FPocket 0.682 · Pocket 2
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 74 / 4744 genomes with a hit
Prevalence 1.6%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt W9BH30

Structure

PDB 6IVO PDB 6IVK PDB 6IVQ PDB 6IVJ PDB 6IVR PDB 6IVM PDB 6IVN PDB 6IVL PDB 6IVP

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MIIRPEQHWFLRLFDWHGSVLSKIIFRLLLNVLMSIIAIISYQWYEQLGIHLTVAPFSLLGIAIAIFLGFRNSASYSRFVEARNLWGTVLIAERTLVRQLRNILPAEHDAHRRIVSYLVAFSWSLKHQLRKTDPTADLRRLLPEERVTEILASSMPTNRILLLAGNEIGQLREAGKLSDITYGLMDNKLDELAHVLGGCERLATTPVPFAYTLILQRTVYLFCTLLPFALVGDLHYMTPFVSVFISYTFLSWDSLAEELEDPFGTAANDLPLNAMCNTIERNLLDMTGQHPLPETLRPDRYFNLT

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

3
  • GO:0005254 Enables the energy-independent facilitated diffusion of a chloride ion through a transmembrane aqueous pore or channel.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0046872 Binding to a metal ion.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

12 records
Show feature table
Start End DB Term Name
43 47 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
2 283 Pfam PF01062 Bestrophin, RFP-TM, chloride channel
2 283 InterPro IPR021134 Bestrophin-like
1 19 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
219 241 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
20 42 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 290 PANTHER PTHR33281 UPF0187 PROTEIN YNEE
6 290 InterPro IPR044669 Voltage-dependent anion channel-forming protein YneE/VCCN1/2-like
48 70 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
71 305 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
48 70 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
20 42 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.805
Likely same site as FPocket 6 4.8 Å 10 shared residues 67% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.408
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Surrounding area
Pocket 3 P2Rank #3
0.369
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Surrounding area
Pocket 4 P2Rank #4
0.31
Likely same site as FPocket 3 1.3 Å 17 shared residues 89% of smaller site
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Surrounding area
Pocket 5 P2Rank #5
0.297
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Surrounding area

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #6
0.958
Likely same site as P2Rank 1 4.8 Å 10 shared residues 67% of smaller site
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Surrounding area
Pocket 2 FPocket #3
0.819
Likely same site as P2Rank 4 1.3 Å 17 shared residues 89% of smaller site
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Surrounding area
Pocket 3 FPocket #8
0.597
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Surrounding area
Pocket 4 FPocket #1
0.56
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Surrounding area
All structural evidence 9 experimental · 1 predicted

Structural evidence

9 + 1

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 6IVO
X-ray 2.45 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Viewing
PDB 6IVK
X-ray 2.65 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVQ
X-ray 2.65 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVJ
X-ray 2.77 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVR
X-ray 2.80 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVM
X-ray 2.95 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVN
X-ray 3.10 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVL
X-ray 3.40 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
PDB 6IVP
X-ray 3.80 Å A,B,C,D,E
96.4% 1-294
PDBe RCSB PDBj File
Loaded
ColabFold VK055_0657
ColabFold — — full sequence — Loaded