Protein profile

VK055_0948

malate dehydrogenase

Genome: KpATCC43816

Gene: AIK79571.1 mqo Structure source: AlphaFold + ColabFold UniProt A0A0H3GT00
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Amino acids 548
Annotations 4
Features 21
PDB binders 0
Druggability 0.999

Overview

Basic information about this protein and its source genome.

Accession
VK055_0948
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK79571.1 mqo
Status
annotated
Amino acids
548
Structure source
AlphaFold + ColabFold
EC
1.1.5.4
GO
GO:0008924 Catalysis of the reaction: (S)-malate + a quinone = oxaloacetate + a quinol. Vitamin K (menaquinone) and several other quinones can act as acceptors. GO:0006099 A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle. GO:0047545 Catalysis of the reaction: (S)-2-hydroxyglutarate + acceptor = 2-oxoglutarate + reduced acceptor.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
88.04

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.999
Structure A0A0H3GT00
Pocket Pocket 1
P2Rank 0.988
Structure A0A0H3GT00
Pocket Pocket 1
ColabFold model
FPocket 0.948 · Pocket 31
P2Rank 0.956 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 174 / 4744 genomes with a hit
Normalized 0.037

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 3 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

3
  • GO:0008924 Catalysis of the reaction: (S)-malate + a quinone = oxaloacetate + a quinol. Vitamin K (menaquinone) and several other quinones can act as acceptors.
  • GO:0006099 A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.
  • GO:0047545 Catalysis of the reaction: (S)-2-hydroxyglutarate + acceptor = 2-oxoglutarate + reduced acceptor.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
134 296 Gene3D G3DSA:3.30.9.10 -
1 23 SignalP_EUK SignalP-noTM SignalP-noTM
24 548 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
4 15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
22 469 PANTHER PTHR43104 L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL
1 23 Phobius SIGNAL_PEPTIDE Signal peptide region
525 548 MobiDBLite mobidb-lite consensus disorder prediction
29 512 NCBIfam TIGR01320 malate dehydrogenase (quinone)
29 512 InterPro IPR006231 Malate:quinone-oxidoreductase
1 21 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
16 23 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
31 284 Gene3D G3DSA:3.50.50.60 -
31 284 InterPro IPR036188 FAD/NAD(P)-binding domain superfamily
1 23 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
21 353 SUPERFAMILY SSF51905 FAD/NAD(P)-binding domain
21 353 InterPro IPR036188 FAD/NAD(P)-binding domain superfamily
28 514 Pfam PF06039 Malate:quinone oxidoreductase (Mqo)
28 514 InterPro IPR006231 Malate:quinone-oxidoreductase
25 514 Hamap MF_00212 Probable malate:quinone oxidoreductase [mqo].
25 514 InterPro IPR006231 Malate:quinone-oxidoreductase
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GT00
AlphaFold — — full sequence — Viewing
ColabFold VK055_0948
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.999
5 0.941

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 24.67 0.9
2 18.76 0.829
3 8.97 0.48
4 3.81 0.148
5 3.74 0.144