KpATCC43816 Protein target profile

membrane AbrB duplication domain protein

Accession: VK055_1141

Gene: AIK79764.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A422WBC3
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Length 338
Pocket druggability (P2Rank · AlphaFold DB model) 0.914
Functional annotation 0 EC 2 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.91, FPocket 0.87). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.87. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 44 of 4744 screened genomes (0.9%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.91) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
0.9% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
85.52 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.914
Structure A0A422WBC3
Pocket Pocket 1
Druggability (FPocket) 0.867
Structure A0A422WBC3
Pocket Pocket 21
ColabFold model
P2Rank 0.943 · Pocket 1
FPocket 0.877 · Pocket 16
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 44 / 4744 genomes with a hit
Prevalence 0.9%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MLLLSVLFSSLLLRIHLPAALLLGPLIAGLILSLRGVKLSIPRPCYLAAQAIVGCMIARAINPSVFGVLFNNWALVLAILLTTLAISGLTGWLLVRYSALPGATGAWGSSPGGASAMVVMAQEYGADVRLVALMQYLRVLFVVGAAALVVRYALGNEAQEMTQDIVWFPSLTLNFPFTLLLTAVACWLGMRLRIPSGAMLLPMLLGALAQGGGWLMLELPEWLLAMAYAVLGWTVGLQFNKAIFLLALKTLPQIIASIIGLILMCALMALALTHILQMDFMTAYLATSPGGLDTVAIIAAGTRADMSFIMALQTLRLFTILLTGPAMARAISRYAPRQ

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

2
  • GO:0010468 Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

38 records
Show feature table
Start End DB Term Name
309 331 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
21 331 Pfam PF05145 Transition state regulatory protein AbrB
21 331 InterPro IPR007820 AbrB family
44 63 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
165 187 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
73 95 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
166 188 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
218 222 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
277 307 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
308 328 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
255 277 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
329 338 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
248 253 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
136 154 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
194 216 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
254 276 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
96 135 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
226 248 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
200 217 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
223 247 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
189 199 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
2 337 PANTHER PTHR38457 REGULATOR ABRB-RELATED
2 337 InterPro IPR007820 AbrB family
1 338 PIRSF PIRSF038991 Protein_AbrB
1 338 InterPro IPR007820 AbrB family
136 155 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
73 95 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 152 NCBIfam TIGR03082 membrane protein AbrB duplication
2 152 InterPro IPR017516 AbrB duplication
177 330 NCBIfam TIGR03082 membrane protein AbrB duplication
177 330 InterPro IPR017516 AbrB duplication
155 165 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
62 72 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
44 61 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
33 43 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.914
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Surrounding area
Pocket 2 P2Rank #2
0.399
Likely same site as FPocket 30 5.0 Å 10 shared residues 100% of smaller site
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Surrounding area
Pocket 3 P2Rank #3
0.175
Likely same site as FPocket 21 1.2 Å 9 shared residues 100% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.159
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Surrounding area
Pocket 5 P2Rank #5
0.079
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #21
0.867
Likely same site as P2Rank 3 1.2 Å 9 shared residues 100% of smaller site
Show in viewer
Surrounding area
Pocket 2 FPocket #22
0.814
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Surrounding area
Pocket 3 FPocket #30
0.337
Likely same site as P2Rank 2 5.0 Å 10 shared residues 100% of smaller site
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Surrounding area
Pocket 4 FPocket #4
0.29
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A422WBC3
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_1141
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A422WBC3