Protein profile

VK055_1226

PTS system, cellobiose-specific IIC component

Genome: KpATCC43816

Gene: AIK79849.1 celB Structure source: AlphaFold + ColabFold UniProt A0A0H3GVY3
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Amino acids 452
Annotations 5
Features 42
PDB binders 0
Druggability 0.626

Overview

Basic information about this protein and its source genome.

Accession
VK055_1226
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK79849.1 celB
Status
annotated
Amino acids
452
Structure source
AlphaFold + ColabFold
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:1902815 The directed movement of N,N'-diacetylchitobiose into a cell or organelle.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
87.389
DEG E-value
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
94.75

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.626
Structure A0A0H3GVY3
Pocket Pocket 19
P2Rank 0.899
Structure A0A0H3GVY3
Pocket Pocket 1
ColabFold model
FPocket 0.435 · Pocket 8
P2Rank 0.901 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 83 / 4744 genomes with a hit
Normalized 0.017

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:1902815 The directed movement of N,N'-diacetylchitobiose into a cell or organelle.

Sequence Features

Domain/signature hits from InterPro and related databases.

42 records
Show feature table
Start End DB Term Name
108 113 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
341 363 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
370 392 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
149 167 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
35 55 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
117 139 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
353 358 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 448 PIRSF PIRSF006351 PTS_EIIC-Cellobiose
1 448 InterPro IPR004796 Phosphotransferase system, cellobiose-type IIC component
407 429 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
224 246 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
145 167 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
404 429 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
8 426 ProSiteProfiles PS51105 PTS_EIIC type-3 domain profile.
8 426 InterPro IPR004501 Phosphotransferase system, EIIC component, type 3
332 352 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
208 226 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
385 403 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
12 440 NCBIfam TIGR00410 PTS system, lactose/cellobiose family IIC component
12 440 InterPro IPR004501 Phosphotransferase system, EIIC component, type 3
302 321 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
82 104 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
30 363 Pfam PF02378 Phosphotransferase system, EIIC
30 363 InterPro IPR003352 Phosphotransferase system, EIIC
12 440 NCBIfam TIGR00359 PTS cellobiose transporter subunit IIC
12 440 InterPro IPR004796 Phosphotransferase system, cellobiose-type IIC component
359 384 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 447 PANTHER PTHR33989 -
31 53 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
114 133 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
301 320 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 34 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
86 107 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
56 85 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
227 247 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
187 207 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
187 209 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
168 186 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
321 331 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
134 144 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
430 452 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
248 300 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GVY3
AlphaFold — — full sequence — Viewing
ColabFold VK055_1226
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
19 0.626
10 0.107
9 0.029
11 0.007

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 29.76 0.899
2 6.29 0.263
3 5.98 0.244
4 5.67 0.227
5 3.58 0.106