Protein profile

VK055_1295

lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase family protein

Genome: KpATCC43816

Gene: lpxL AIK79918.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GLM3
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Amino acids 304
Annotations 9
Features 18
PDB binders 0
Druggability 0.737

Overview

Basic information about this protein and its source genome.

Accession
VK055_1295
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
lpxL AIK79918.1
Status
annotated
Amino acids
304
Structure source
AlphaFold + ColabFold
EC
2.3.1.242
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0036104 The chemical reactions and pathways resulting in the formation of Kdo2-lipid A, a lipopolysaccharide (LPS) component. GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2. GO:0009409 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism. GO:0008951 Catalysis of the reaction: a palmitoleoyl-[acyl-carrier protein] + alpha-KDO-(2->4)-alpha-KDO-(2->6)-lipid IVA = KDO2-(palmitoleoyl)-lipid IVA + a holo-[acyl-carrier protein]. GO:0009245 The chemical reactions and pathways resulting in the formation of lipid A, the glycolipid group of bacterial lipopolysaccharides, consisting of four to six fatty acyl chains linked to two glucosamine residues. Further modifications of the backbone are common.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
52.333
DEG E-value
2.59e-122
Localization
CytoplasmicMembrane
ColabFold pLDDT
96.37

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.737
Structure A0A0H3GLM3
Pocket Pocket 1
P2Rank 0.882
Structure A0A0H3GLM3
Pocket Pocket 1
ColabFold model
FPocket 0.812 · Pocket 1
P2Rank 0.825 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 114 / 4744 genomes with a hit
Normalized 0.024

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0036104 The chemical reactions and pathways resulting in the formation of Kdo2-lipid A, a lipopolysaccharide (LPS) component.
  • GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
  • GO:0009409 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism.
  • GO:0008951 Catalysis of the reaction: a palmitoleoyl-[acyl-carrier protein] + alpha-KDO-(2->4)-alpha-KDO-(2->6)-lipid IVA = KDO2-(palmitoleoyl)-lipid IVA + a holo-[acyl-carrier protein].
  • GO:0009245 The chemical reactions and pathways resulting in the formation of lipid A, the glycolipid group of bacterial lipopolysaccharides, consisting of four to six fatty acyl chains linked to two glucosamine residues. Further modifications of the backbone are common.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0009103 The chemical reactions and pathways resulting in the formation of lipopolysaccharides, any of a group of related, structurally complex components of the outer membrane of Gram-negative bacteria.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
2 304 Hamap MF_01942 Lipid A biosynthesis lauroyltransferase [lpxL].
2 304 InterPro IPR011920 Lipid A biosynthesis lauroyl/palmitoleoyl acyltransferase
4 304 NCBIfam TIGR02207 LpxL/LpxP family Kdo(2)-lipid IV(A) lauroyl/palmitoleoyl acyltransferase
4 304 InterPro IPR011920 Lipid A biosynthesis lauroyl/palmitoleoyl acyltransferase
1 304 PIRSF PIRSF026649 MsbB
1 304 InterPro IPR004960 Bacterial lipid A biosynthesis acyltransferase
1 14 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
103 294 CDD cd07984 LPLAT_LABLAT-like
103 294 InterPro IPR004960 Bacterial lipid A biosynthesis acyltransferase
4 295 Pfam PF03279 Bacterial lipid A biosynthesis acyltransferase
4 295 InterPro IPR004960 Bacterial lipid A biosynthesis acyltransferase
39 304 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
5 300 PANTHER PTHR30606 LIPID A BIOSYNTHESIS LAUROYL ACYLTRANSFERASE
5 300 InterPro IPR004960 Bacterial lipid A biosynthesis acyltransferase
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 38 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 304 Hamap MF_01943 Lipid A biosynthesis palmitoleoyltransferase [lpxP].
1 304 InterPro IPR030857 Lipid A biosynthesis palmitoleoyltransferase, LpxP

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GLM3
AlphaFold — — full sequence — Viewing
ColabFold VK055_1295
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.737
15 0.363

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 16.88 0.79
2 4.43 0.187
3 2.69 0.08
4 2.44 0.066