KpATCC43816 Protein target profile

aspT/YidE/YbjL antiporter duplication domain protein

Accession: VK055_1611

Gene: AIK80231.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A6T6U3
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Length 561
Pocket druggability (P2Rank · AlphaFold DB model) 0.594
Functional annotation 0 EC 2 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Second pocket predictor agrees P2Rank predicted binding-site probability 0.59. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 106 of 4744 screened genomes (2.2%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.59) — check the ligand evidence before deprioritizing on off-target risk alone. Best pocket may be a modeling artifact FPocket selected-pocket score 0.91, but this pocket's geometry is unusually large/diffuse compared to this protein's other predicted pockets — may reflect a low-confidence or disordered model region rather than a real cavity.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.2% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
23.274 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
85.26 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.594
Structure A6T6U3
Pocket Pocket 1
Druggability (FPocket) 0.911
Structure A6T6U3
Pocket Pocket 2
ColabFold model
P2Rank 0.73 · Pocket 1
FPocket 0.699 · Pocket 38
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 106 / 4744 genomes with a hit
Prevalence 2.2%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MNINVADLLNGNYILLLFVVLALGLCLGKLRLGSVQLGNSIGVLVVSLLLGQQHFAINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGSAMLIAMVLGKVFGWDIGLTAGMLAGAMTSTPVLVGAGDTLRHFGLPSDQLAQSLDHLSLGYALTYLVGLVSLIVGARYMPKLQHQDLQTSAQQIARERGLDTDSKRKVYLPVIRAYRVGPELVAWADGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDDIALVGYPDAHARLDPSFRNGKEVFDRDLLDMRIVTEEIVVKNHNAVGRRLAQLKLTDHGCFLNRVIRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTVADRIGFISIHSQVTDLLAFCAFFIVGLMIGMITFQFSSFSFGIGNAAGLLFAGIMLGFLRANHPTFGYIPQGALNMVKEFGLMVFMAGVGLSAGAGINNGLGAVGGQMLAAGLIVSLVPVVICFLFGAYVLRMNRAMLFGAMMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTLIVIIWPGLQ

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

2
  • GO:0006813 The directed movement of potassium ions (K+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0008324 Enables the transfer of cation from one side of a membrane to the other.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

58 records
Show feature table
Start End DB Term Name
224 286 FunFam G3DSA:3.30.70.1450:FF:000003 Putative transport protein YbjL
401 405 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
21 559 PANTHER PTHR30445 UNCHARACTERIZED
12 30 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
92 114 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
159 178 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
64 86 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
93 115 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
532 557 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 557 Hamap MF_01015 Putative transport protein YbjL [ybjL].
1 557 InterPro IPR023017 Transport protein YbjL, putative
535 557 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
292 373 ProSiteProfiles PS51202 RCK C-terminal domain profile.
292 373 InterPro IPR006037 Regulator of K+ conductance, C-terminal
227 282 SUPERFAMILY SSF116726 TrkA C-terminal domain-like
227 282 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
31 36 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
182 379 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
558 561 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
380 400 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
385 554 Pfam PF06826 Predicted Permease Membrane Region
385 554 InterPro IPR006512 YidE/YbjL duplication
17 181 Pfam PF06826 Predicted Permease Membrane Region
17 181 InterPro IPR006512 YidE/YbjL duplication
61 85 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
427 446 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
383 402 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
202 288 ProSiteProfiles PS51202 RCK C-terminal domain profile.
202 288 InterPro IPR006037 Regulator of K+ conductance, C-terminal
476 498 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
305 371 Pfam PF02080 TrkA-C domain
305 371 InterPro IPR006037 Regulator of K+ conductance, C-terminal
227 282 Pfam PF02080 TrkA-C domain
227 282 InterPro IPR006037 Regulator of K+ conductance, C-terminal
37 55 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
406 426 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
21 168 NCBIfam TIGR01625 AspT/YidE/YbjL antiporter duplication domain
21 168 InterPro IPR006512 YidE/YbjL duplication
391 541 NCBIfam TIGR01625 AspT/YidE/YbjL antiporter duplication domain
391 541 InterPro IPR006512 YidE/YbjL duplication
161 181 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
406 428 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
223 286 Gene3D G3DSA:3.30.70.1450 -
223 286 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
56 60 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
447 464 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
86 91 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
476 498 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
465 475 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
499 531 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
115 160 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
304 373 Gene3D G3DSA:3.30.70.1450 -
304 373 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
291 374 SUPERFAMILY SSF116726 TrkA C-terminal domain-like
291 374 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
449 471 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.594
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Surrounding area
Pocket 2 P2Rank #2
0.541
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Surrounding area
Pocket 3 P2Rank #3
0.502
Likely same site as FPocket 23 0.1 Å 15 shared residues 88% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.349
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Surrounding area
Pocket 5 P2Rank #5
0.239
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #2
0.911 Unusual size
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Surrounding area
Pocket 2 FPocket #34
0.814
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Surrounding area
Pocket 3 FPocket #23
0.352
Likely same site as P2Rank 3 0.1 Å 15 shared residues 88% of smaller site
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Surrounding area
Pocket 4 FPocket #30
0.323
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A6T6U3
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_1611
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A6T6U3