KpATCC43816 Protein target profile

sulfatase family protein

Accession: VK055_1684

Gene: AIK80304.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GKE6
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Length 526
Pocket druggability (P2Rank · AlphaFold DB model) 0.967
Functional annotation 0 EC 5 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Second pocket predictor agrees P2Rank predicted binding-site probability 0.97. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 65 of 4744 screened genomes (1.4%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.97) — check the ligand evidence before deprioritizing on off-target risk alone. Best pocket may be a modeling artifact FPocket selected-pocket score 0.93, but this pocket's geometry is unusually large/diffuse compared to this protein's other predicted pockets — may reflect a low-confidence or disordered model region rather than a real cavity.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
1.4% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
39.041 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
90.95 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.967
Structure A0A0H3GKE6
Pocket Pocket 1
Druggability (FPocket) 0.932
Structure A0A0H3GKE6
Pocket Pocket 32
ColabFold model
P2Rank 0.96 · Pocket 1
FPocket 0.787 · Pocket 9
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 65 / 4744 genomes with a hit
Prevalence 1.4%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GKE6

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MNVTTLKDTLVARRLALNPWTGFYFLQSLLINLALGYEFSLLYTVAFTCVLHLLWRAFPRVQKAVVGAYSLLAALYYPFGQAYGAPNFNTLLALHATNVEESTEILTIFPWYNYLLAVFIFALGVIAVRRRIVEPSRWGKMDTLGLMFSIGIFFLQPVQNLAWGGVFKVIDTGYPAFRFVKDVVVNNNEVLDEQARMAQLAGMKDSWHVLAVKPKYHLYVVVIGESARRDALGAFGGHWDNTPFASSVNGYLFNNYIAASGSTQKSLGLTLNRVVDGKPQYQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVQFLKNGDFEANKNTQDEQLLKLTEQVLSTQRTQPQLIVLHLMGSHPQACDRTKGKYTVFVQSKETSCYLYSMTQTDSLLAKLYHQLQNSGDTFSLTYFSDHGLAFKERGKEVQYLAHDDKFQQNFQVPFMVLSSDDKAHKVIKAQRSANDFLSFFSQWTGIQAAEITPRYRFISEQKAGPVYITNFQLQKVDYAHLGTDEFTVN

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

5
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0008484 Catalysis of the reaction: RSO-R' + H2O = RSOOH + R'H. This reaction is the hydrolysis of a sulfuric ester bond, an ester formed from sulfuric acid, O=SO(OH)2.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016776 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to a phosphate group (acceptor).
  • GO:0009244 The chemical reactions and pathways resulting in the formation of the core region of bacterial lipopolysaccharides, which contains ten saccharide residues.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

22 records
Show feature table
Start End DB Term Name
61 79 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
64 86 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
215 484 CDD cd16017 LptA
1 28 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
55 60 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
145 167 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
141 158 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
33 55 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
220 487 SUPERFAMILY SSF53649 Alkaline phosphatase-like
220 487 InterPro IPR017850 Alkaline-phosphatase-like, core domain superfamily
209 507 Gene3D G3DSA:3.40.720.10 Alkaline Phosphatase, subunit A
209 507 InterPro IPR017850 Alkaline-phosphatase-like, core domain superfamily
106 128 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
130 140 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
159 526 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
111 129 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
29 54 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
219 483 Pfam PF00884 Sulfatase
219 483 InterPro IPR000917 Sulfatase, N-terminal
80 110 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
11 503 PANTHER PTHR30443 INNER MEMBRANE PROTEIN
11 503 InterPro IPR040423 Phosphoethanolamine transferase

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.967
Likely same site as FPocket 32 7.4 Å 15 shared residues 79% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.617
Likely same site as FPocket 23 2.0 Å 15 shared residues 88% of smaller site
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Surrounding area
Pocket 3 P2Rank #3
0.376
Likely same site as FPocket 6 3.2 Å 11 shared residues 100% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.212
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Surrounding area
Pocket 5 P2Rank #5
0.116
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #32
0.932 Unusual size
Likely same site as P2Rank 1 7.4 Å 15 shared residues 79% of smaller site
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Surrounding area
Pocket 2 FPocket #6
0.818 Unusual size
Likely same site as P2Rank 3 3.2 Å 11 shared residues 100% of smaller site
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Surrounding area
Pocket 3 FPocket #23
0.303 Unusual size
Likely same site as P2Rank 2 2.0 Å 15 shared residues 88% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GKE6
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_1684
ColabFold — — full sequence — Loaded