Protein profile

VK055_2105

acrA Membrane Fusion Protein

Genome: KpATCC43816

Gene: acrA AIK80710.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GP47
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Amino acids 397
Annotations 7
Features 25
PDB binders 0
Druggability 0.448

Overview

Basic information about this protein and its source genome.

Accession
VK055_2105
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
acrA AIK80710.1
Status
annotated
Amino acids
397
Structure source
AlphaFold + ColabFold
GO
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
85.025
DEG E-value
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
84.42

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.448
Structure A0A0H3GP47
Pocket Pocket 15
P2Rank 0.002
Structure A0A0H3GP47
Pocket Pocket 1
ColabFold model
FPocket 0.532 · Pocket 10
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 146 / 4744 genomes with a hit
Normalized 0.031

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
  • GO:0140330 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell through the outer membrane.

Sequence Features

Domain/signature hits from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
52 300 SUPERFAMILY SSF111369 HlyD-like secretion proteins
1 5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
6 16 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
284 376 Gene3D G3DSA:2.40.420.20 -
378 397 MobiDBLite mobidb-lite consensus disorder prediction
99 172 FunFam G3DSA:1.10.287.470:FF:000002 Efflux RND transporter periplasmic adaptor subunit
204 284 FunFam G3DSA:2.40.30.170:FF:000001 Multidrug resistance efflux transporter MdtE
375 397 MobiDBLite mobidb-lite consensus disorder prediction
1 21 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
22 397 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 21 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
99 172 Gene3D G3DSA:1.10.287.470 Helix hairpin bin
1 387 PANTHER PTHR30158 ACRA/E-RELATED COMPONENT OF DRUG EFFLUX TRANSPORTER
64 208 Gene3D G3DSA:2.40.50.100 -
40 375 NCBIfam TIGR01730 efflux RND transporter periplasmic adaptor subunit
40 375 InterPro IPR006143 RND efflux pump, membrane fusion protein
284 376 FunFam G3DSA:2.40.420.20:FF:000001 Efflux RND transporter periplasmic adaptor subunit
58 283 Gene3D G3DSA:2.40.30.170 -
63 293 Pfam PF16576 Barrel-sandwich domain of CusB or HlyD membrane-fusion
63 293 InterPro IPR032317 RND efflux pump, membrane fusion protein, barrel-sandwich domain
1 25 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
45 360 Pfam PF00529 Cation efflux system protein CusB domain 1
45 360 InterPro IPR043602 Cation efflux system protein CusB, domain 1
17 21 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 21 Phobius SIGNAL_PEPTIDE Signal peptide region

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GP47
AlphaFold — — full sequence — Viewing
ColabFold VK055_2105
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
7 0.021
14 0.015
19 0.008
2 0.002

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 0.77 0.002