Protein profile

VK055_2179

tRNA sulfurtransferase ThiI

Genome: KpATCC43816

Gene: thiI AIK80784.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GKE9
Export view
Amino acids 482
Annotations 13
Features 32
PDB binders 0
Druggability 0.958

Overview

Basic information about this protein and its source genome.

Accession
VK055_2179
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
thiI AIK80784.1
Status
annotated
Amino acids
482
Structure source
AlphaFold + ColabFold
EC
2.8.1.4
GO
GO:0016783 Catalysis of the transfer of sulfur atoms from one compound (donor) to another (acceptor). GO:0004810 Catalysis of the reaction: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate. GO:0034227 The addition a sulfur atom to a nucleotide in a tRNA molecule. GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0052837 The chemical reactions and pathways resulting in the formation of a thiazole, a five-membered heterocyclic ring structure containing a sulfur in the 1-position and a nitrogen in the 3-position. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
28.889
Localization
Cytoplasmic
ColabFold pLDDT
91.09

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.958
Structure A0A0H3GKE9
Pocket Pocket 1
P2Rank 0.918
Structure A0A0H3GKE9
Pocket Pocket 1
ColabFold model
FPocket 0.658 · Pocket 24
P2Rank 0.905 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 156 / 4744 genomes with a hit
Normalized 0.033

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 12 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

12
  • GO:0016783 Catalysis of the transfer of sulfur atoms from one compound (donor) to another (acceptor).
  • GO:0004810 Catalysis of the reaction: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate.
  • GO:0034227 The addition a sulfur atom to a nucleotide in a tRNA molecule.
  • GO:0003723 Binding to an RNA molecule or a portion thereof.
  • GO:0052837 The chemical reactions and pathways resulting in the formation of a thiazole, a five-membered heterocyclic ring structure containing a sulfur in the 1-position and a nitrogen in the 3-position.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0140741 Catalyzes the reaction: ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.
  • GO:0009228 The chemical reactions and pathways resulting in the formation of thiamine (vitamin B1), a water soluble vitamin present in fresh vegetables and meats, especially liver.
  • GO:0009229 The chemical reactions and pathways resulting in the formation of thiamine diphosphate, a derivative of thiamine (vitamin B1) which acts as a coenzyme in a range of processes including the Krebs cycle.
  • GO:0002937 The processes whereby a uridine residue in a tRNA is converted to 4-thiouridine. Typically 4-thiouridine is found at position 8, in many transfer RNAs.

Sequence Features

Domain/signature hits from InterPro and related databases.

32 records
Show feature table
Start End DB Term Name
1 482 Hamap MF_00021 tRNA sulfurtransferase [thiI].
1 482 InterPro IPR003720 tRNA sulfurtransferase ThiI
397 482 FunFam G3DSA:3.40.250.10:FF:000003 tRNA sulfurtransferase
61 165 ProSiteProfiles PS51165 THUMP domain profile.
61 165 InterPro IPR004114 THUMP domain
174 372 SUPERFAMILY SSF52402 Adenine nucleotide alpha hydrolases-like
1 399 PANTHER PTHR43209 TRNA SULFURTRANSFERASE
432 480 ProSiteProfiles PS50206 Rhodanese domain profile.
432 480 InterPro IPR001763 Rhodanese-like domain
1 174 FunFam G3DSA:3.30.2130.30:FF:000002 tRNA sulfurtransferase
175 369 Pfam PF02568 Thiamine biosynthesis protein (ThiI)
175 369 InterPro IPR020536 Thil, AANH domain
175 395 Gene3D G3DSA:3.40.50.620 HUPs
175 395 InterPro IPR014729 Rossmann-like alpha/beta/alpha sandwich fold
1 174 Gene3D G3DSA:3.30.2130.30 -
175 396 FunFam G3DSA:3.40.50.620:FF:000029 tRNA sulfurtransferase
396 482 Gene3D G3DSA:3.40.250.10 -
396 482 InterPro IPR036873 Rhodanese-like domain superfamily
28 161 Pfam PF02926 THUMP domain
28 161 InterPro IPR004114 THUMP domain
75 165 SMART SM00981 THUMP_a_2
75 165 InterPro IPR004114 THUMP domain
2 170 CDD cd11716 THUMP_ThiI
1 173 SUPERFAMILY SSF143437 THUMP domain-like
407 481 SUPERFAMILY SSF52821 Rhodanese/Cell cycle control phosphatase
407 481 InterPro IPR036873 Rhodanese-like domain superfamily
382 482 NCBIfam TIGR04271 thiazole biosynthesis domain
382 482 InterPro IPR026340 Thiazole biosynthesis domain
180 353 CDD cd01712 ThiI
180 353 InterPro IPR020536 Thil, AANH domain
4 377 NCBIfam TIGR00342 tRNA 4-thiouridine(8) synthase ThiI
4 377 InterPro IPR003720 tRNA sulfurtransferase ThiI

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GKE9
AlphaFold — — full sequence — Viewing
ColabFold VK055_2179
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.958
10 0.704

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 17.07 0.795
2 4.21 0.172
3 2.1 0.048
4 1.75 0.032