Protein profile

VK055_2215

phosphate regulon sensor kinase PhoR

Genome: KpATCC43816

Gene: phoR AIK80820.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GNY2
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Amino acids 431
Annotations 14
Features 51
PDB binders 2
Druggability 0.913

Overview

Basic information about this protein and its source genome.

Accession
VK055_2215
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
phoR AIK80820.1
Status
annotated
Amino acids
431
Structure source
AlphaFold + ColabFold
EC
2.7.13.3
GO
GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0004721 Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. Together with protein kinases, these enzymes control the state of phosphorylation of cellular proteins and thereby provide an important mechanism for regulating cellular activity. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
86.744
DEG E-value
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
88.14

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.913
Structure A0A0H3GNY2
Pocket Pocket 7
P2Rank 0.947
Structure A0A0H3GNY2
Pocket Pocket 1
ColabFold model
FPocket 0.847 · Pocket 38
P2Rank 0.929 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 124 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 13 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

13
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0004721 Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. Together with protein kinases, these enzymes control the state of phosphorylation of cellular proteins and thereby provide an important mechanism for regulating cellular activity.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0004673 Catalysis of the reaction: ATP + protein L-histidine = ADP + protein phospho-L-histidine.
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0016036 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of phosphate.
  • GO:0006817 The directed movement of phosphate ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence Features

Domain/signature hits from InterPro and related databases.

51 records
Show feature table
Start End DB Term Name
86 199 FunFam G3DSA:3.30.450.20:FF:000044 Phosphate regulon sensor histidine kinase PhoR
313 424 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
313 424 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
190 268 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
190 268 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
99 194 Pfam PF00989 PAS fold
99 194 InterPro IPR013767 PAS fold
86 199 Gene3D G3DSA:3.30.450.20 PAS domain
275 428 FunFam G3DSA:3.30.565.10:FF:000032 Phosphate regulon sensor histidine kinase PhoR
210 425 ProSiteProfiles PS50109 Histidine kinase domain profile.
210 425 InterPro IPR005467 Histidine kinase domain
29 33 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
107 213 CDD cd00130 PAS
107 213 InterPro IPR000014 PAS domain
409 422 PRINTS PR00344 Bacterial sensor protein C-terminal signature
409 422 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
368 378 PRINTS PR00344 Bacterial sensor protein C-terminal signature
368 378 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
385 403 PRINTS PR00344 Bacterial sensor protein C-terminal signature
385 403 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
350 364 PRINTS PR00344 Bacterial sensor protein C-terminal signature
350 364 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
96 420 NCBIfam TIGR02966 phosphate regulon sensor histidine kinase PhoR
96 420 InterPro IPR014310 Signal transduction histidine kinase, phosphate regulon sensor PhoR
60 431 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
200 268 Gene3D G3DSA:1.10.287.130 -
203 269 SMART SM00388 HisKA_10
203 269 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
203 265 CDD cd00082 HisKA
203 265 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
12 34 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
318 425 CDD cd16952 HATPase_EcPhoR-like
313 425 SMART SM00387 HKATPase_4
313 425 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
199 269 FunFam G3DSA:1.10.287.130:FF:000001 Two-component sensor histidine kinase
87 195 SUPERFAMILY SSF55785 PYP-like sensor domain (PAS domain)
87 195 InterPro IPR035965 PAS domain superfamily
12 28 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 427 PANTHER PTHR45453 PHOSPHATE REGULON SENSOR PROTEIN PHOR
259 423 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
259 423 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
34 59 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
6 91 Pfam PF11808 Phosphate regulon sensor protein PhoR
6 91 InterPro IPR021766 Phosphate regulon sensor protein PhoR
98 166 SMART SM00091 pas_2
98 166 InterPro IPR000014 PAS domain
204 268 Pfam PF00512 His Kinase A (phospho-acceptor) domain
204 268 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
278 428 Gene3D G3DSA:3.30.565.10 -
278 428 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GNY2
AlphaFold — — full sequence — Viewing
ColabFold VK055_2215
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
7 0.913

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.7 0.883
2 2.68 0.079
3 1.27 0.013
4 1.24 0.012
5 1.11 0.008

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.