KpATCC43816 Protein target profile

protein-P-II uridylyltransferase

Accession: VK055_2385

Gene: AIK80982.1 glnD 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GNI7
Export view
Length 867
Pocket druggability (P2Rank · AlphaFold DB model) 0.823
Functional annotation 0 EC 6 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Moderate predicted binding pocket FPocket selected-pocket score 0.59. This supports review but is not experimental validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.82. Review the complete evidence below

Risks to review

Microbiome similarity 122 of 4744 screened genomes (2.6%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.82) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.6% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
88.671 Higher values support similarity to known essential genes.
DEG E-value
0.0 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
92.04 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.823
Structure A0A0H3GNI7
Pocket Pocket 1
Druggability (FPocket) 0.586
Structure A0A0H3GNI7
Pocket Pocket 9
ColabFold model
P2Rank 0.86 · Pocket 1
FPocket 0.436 · Pocket 9
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 122 / 4744 genomes with a hit
Prevalence 2.6%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MSWPQGDLNCAAIKAHIDTFQHWLGEAFDSGIVAEQLIAARTEFIDQLLQRLWIACGFESVSDLALVAVGGYGRGELHPLSDVDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECLLEGLSDLTVATNLIESRLLIGDVALFLELQKHIFSDGFWPSEKFFAAKVEEQNVRHQRYHGTSYNLEPDVKSSPGGLRDIHTLQWVARRHFGATSMDEMVGFGFLTEAERNELNECLHQLWRIRFALHLELTRYDNRLLFDRQLSVARRLGYEGDGNQPIEHMMKDFFRVTRRVSELNQMLLQLFEEAILALTEDEKPRPIDDDFQLRGTLIDLRDDTLFIREPQAILRMFYIMVRNSTITGIYSTTLRHLRHARRHLTQPLCYIPEARTLFLSMLRHQGAVSRGLLPMHRHSVLWAYMPQWSHIVGQMQFDLFHAYTVDEHTVRVMLKLESFAKEETRSRHPLCVELWPRLTHPELILIAALFHDIAKGRGGDHSILGAQDVLKFAELHGLNSRETQLVAWLVRHHLLMSVTAQRRDIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQLRRGMQSTPDMRERVRHHQLQALALLRMDNINEEALHQIWNRCRANYFVRHTPTQLAWHARNLLRHDLNNPMILLSSQATRGGTEIFIWSPDRPYLFAAVCGELDRRNLSVHDAQIFTTRDGMAMDTFIVLEPDGSPLSADRHEAIRQGLEQTITQRSWQPPAPRRQAAKLRHFSVDTEVNFLPTHTDRKSFLELIALDQPGLLARVGQVFADLGISLHGARITTIGERVEDLFIIATADRRALNNELQQEVQQRLTAALNPNDKG

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Subcellular localization

Localization
Cytoplasmic

Gene Ontology (GO)

6
  • GO:0008773 Catalysis of the reaction: UTP + (protein-PII) = diphosphate + uridylyl-(protein-PII).
  • GO:0016779 Catalysis of the transfer of a nucleotidyl group from one compound (donor) to another (acceptor).
  • GO:0006807 OBSOLETE. The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.
  • GO:0008893 Catalysis of the reaction: guanosine 3',5'-bis(diphosphate) + H2O = diphosphate + GDP + H+.
  • GO:0008081 Catalysis of the hydrolysis of a phosphodiester to give a phosphomonoester and a free hydroxyl group.
  • GO:0006808 Any process that modulates the frequency, rate or extent of nitrogen utilization.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

41 records
Show feature table
Start End DB Term Name
793 867 ProSiteProfiles PS51671 ACT domain profile.
793 867 InterPro IPR002912 ACT domain
686 761 ProSiteProfiles PS51671 ACT domain profile.
686 761 InterPro IPR002912 ACT domain
1 867 Hamap MF_00277 Bifunctional uridylyltransferase/uridylyl-removing enzyme [glnD].
1 867 InterPro IPR010043 Bifunctional uridylyltransferase/uridylyl-removing enzyme
684 755 CDD cd04900 ACT_UUR-like_1
682 755 SUPERFAMILY SSF55021 ACT-like
682 755 InterPro IPR045865 ACT-like domain
3 866 PIRSF PIRSF006288 PII_uridyltransf
3 866 InterPro IPR010043 Bifunctional uridylyltransferase/uridylyl-removing enzyme
792 850 SUPERFAMILY SSF55021 ACT-like
792 850 InterPro IPR045865 ACT-like domain
443 595 CDD cd00077 HDc
443 595 InterPro IPR003607 HD/PDEase domain
794 851 Pfam PF01842 ACT domain
794 851 InterPro IPR002912 ACT domain
686 739 Pfam PF01842 ACT domain
686 739 InterPro IPR002912 ACT domain
434 576 Gene3D G3DSA:1.10.3210.10 Hypothetical protein af1432
57 124 Pfam PF01909 Nucleotidyltransferase domain
57 124 InterPro IPR002934 Polymerase, nucleotidyl transferase domain
24 160 CDD cd05401 NT_GlnE_GlnD_like
794 861 CDD cd04899 ACT_ACR-UUR-like_2
8 865 PANTHER PTHR47320 BIFUNCTIONAL URIDYLYLTRANSFERASE/URIDYLYL-REMOVING ENZYME
8 865 InterPro IPR010043 Bifunctional uridylyltransferase/uridylyl-removing enzyme
441 588 SMART SM00471 hd_13
441 588 InterPro IPR003607 HD/PDEase domain
189 305 SUPERFAMILY SSF81593 Nucleotidyltransferase substrate binding subunit/domain
445 578 Pfam PF01966 HD domain
445 578 InterPro IPR006674 HD domain
434 577 SUPERFAMILY SSF109604 HD-domain/PDEase-like
35 165 SUPERFAMILY SSF81301 Nucleotidyltransferase
35 165 InterPro IPR043519 Nucleotidyltransferase superfamily
445 567 ProSiteProfiles PS51831 HD domain profile.
445 567 InterPro IPR006674 HD domain
170 308 Pfam PF08335 GlnD PII-uridylyltransferase
170 308 InterPro IPR013546 PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase
32 861 NCBIfam TIGR01693 [protein-PII] uridylyltransferase
32 861 InterPro IPR010043 Bifunctional uridylyltransferase/uridylyl-removing enzyme
434 576 FunFam G3DSA:1.10.3210.10:FF:000005 Bifunctional uridylyltransferase/uridylyl-removing enzyme

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

Download VMD script Full viewer

Loading 3D structure...

Drag to rotate — click the view, then scroll to zoom.

Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.823
Likely same site as FPocket 63 1.2 Å 18 shared residues 95% of smaller site
Show in viewer
Surrounding area
Pocket 2 P2Rank #2
0.789
Show in viewer
Surrounding area
Pocket 3 P2Rank #3
0.755
Show in viewer
Surrounding area
Pocket 4 P2Rank #4
0.526
Show in viewer
Surrounding area
Pocket 5 P2Rank #5
0.486
Show in viewer
Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #9
0.586
Show in viewer
Surrounding area
Pocket 2 FPocket #10
0.481
Show in viewer
Surrounding area
Pocket 3 FPocket #8
0.356
Show in viewer
Surrounding area
Pocket 4 FPocket #63
0.338
Likely same site as P2Rank 1 1.2 Å 18 shared residues 95% of smaller site
Show in viewer
Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GNI7
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_2385
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GNI7