Protein profile

VK055_2405

pullulanase

Genome: KpATCC43816

Gene: AIK81002.1 pulA Structure source: AlphaFold + ColabFold UniProt A0A0H3GJK2
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Amino acids 1102
Annotations 5
Features 44
PDB binders 1
Druggability 0.302

Overview

Basic information about this protein and its source genome.

Accession
VK055_2405
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK81002.1 pulA
Status
annotated
Amino acids
1102
Structure source
AlphaFold + ColabFold
EC
3.2.1.41
GO
GO:0030246 Binding to a carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates. GO:0051060 Catalysis of the hydrolysis of (1,6)-alpha-D-glucosidic linkages in pullulan (a linear polymer of alpha-(1,6)-linked maltotriose units) and in amylopectin and glycogen, and the a- and b-limit dextrins of amylopectin and glycogen. GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
29.058
Localization
Unknown
ColabFold pLDDT
94.54

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.302
Structure A0A0H3GJK2
Pocket Pocket 10
P2Rank 0.818
Structure A0A0H3GJK2
Pocket Pocket 1
ColabFold model
FPocket 0.341 · Pocket 57
P2Rank 0.882 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 14 / 4744 genomes with a hit
Normalized 0.003

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0030246 Binding to a carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates.
  • GO:0051060 Catalysis of the hydrolysis of (1,6)-alpha-D-glucosidic linkages in pullulan (a linear polymer of alpha-(1,6)-linked maltotriose units) and in amylopectin and glycogen, and the a- and b-limit dextrins of amylopectin and glycogen.
  • GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond.

Sequence Features

Domain/signature hits from InterPro and related databases.

44 records
Show feature table
Start End DB Term Name
71 183 CDD cd10315 CBM41_pullulanase
71 183 InterPro IPR005323 Pullulanase, carbohydrate-binding module 41
18 26 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
52 180 SUPERFAMILY SSF49452 Starch-binding domain-like
52 180 InterPro IPR013784 Carbohydrate-binding-like fold
27 1102 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1 25 SignalP_EUK SignalP-noTM SignalP-noTM
1 20 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
412 984 SUPERFAMILY SSF51445 (Trans)glycosidases
412 984 InterPro IPR017853 Glycoside hydrolase superfamily
925 1085 Pfam PF11852 Alpha-1,6-glucosidases, pullulanase-type, C-terminal
925 1085 InterPro IPR024561 Alpha-1,6-glucosidases, pullulanase-type, C-terminal
545 864 SMART SM00642 aamy
545 864 InterPro IPR006047 Glycosyl hydrolase, family 13, catalytic domain
316 418 SUPERFAMILY SSF81296 E set domains
316 418 InterPro IPR014756 Immunoglobulin E-set
6 17 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
192 306 Gene3D G3DSA:2.60.40.1130 -
316 400 Pfam PF02922 Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
316 400 InterPro IPR004193 Glycoside hydrolase, family 13, N-terminal
1 26 Phobius SIGNAL_PEPTIDE Signal peptide region
185 305 SUPERFAMILY SSF81296 E set domains
185 305 InterPro IPR014756 Immunoglobulin E-set
249 1042 PANTHER PTHR43002 GLYCOGEN DEBRANCHING ENZYME
25 50 MobiDBLite mobidb-lite consensus disorder prediction
89 180 Pfam PF03714 Bacterial pullanase-associated domain
89 180 InterPro IPR005323 Pullulanase, carbohydrate-binding module 41
501 574 Pfam PF18494 Pullulanase Ins domain
501 574 InterPro IPR041111 Pullulanase, Ins domain
193 1089 NCBIfam TIGR02103 pullulanase-type alpha-1,6-glucosidase
193 1089 InterPro IPR011839 Alpha-1,6-glucosidases, pullulanase-type
994 1102 Gene3D G3DSA:2.60.40.1180 -
994 1102 InterPro IPR013780 Glycosyl hydrolase, all-beta
1 26 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
196 305 Pfam PF17967 Pullulanase N2 domain
196 305 InterPro IPR040671 Pullulanase, N2 domain
415 993 Gene3D G3DSA:3.20.20.80 Glycosidases
307 414 Gene3D G3DSA:2.60.40.10 Immunoglobulins
307 414 InterPro IPR013783 Immunoglobulin-like fold
443 931 CDD cd11341 AmyAc_Pullulanase_LD-like
986 1101 SUPERFAMILY SSF51011 Glycosyl hydrolase domain
51 182 Gene3D G3DSA:2.60.40.1110 -
317 415 CDD cd02860 E_set_Pullulanase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GJK2
AlphaFold — — full sequence — Viewing
ColabFold VK055_2405
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
10 0.302

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 14.87 0.737
2 7.9 0.419
3 3.78 0.146
4 3.1 0.104
5 2.71 0.081

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

2 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
TAM
6JFJ
A0A0C5GWS2 163.2 Da LogP -1.17 TPSA 86.7 ✓ Ro5 ✓ Clean C(CO)C(CCO)(CCO)N

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.