Protein profile

VK055_2416

type II secretion system protein L

Genome: KpATCC43816

Gene: AIK81013.1 gspL Structure source: AlphaFold + ColabFold UniProt A0A0H3GNF4
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Amino acids 387
Annotations 4
Features 15
PDB binders 0
Druggability 0.733

Overview

Basic information about this protein and its source genome.

Accession
VK055_2416
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK81013.1 gspL
Status
annotated
Amino acids
387
Structure source
AlphaFold + ColabFold
GO
GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
27.32
Localization
Unknown
ColabFold pLDDT
84.92

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.733
Structure A0A0H3GNF4
Pocket Pocket 11
P2Rank 0.049
Structure A0A0H3GNF4
Pocket Pocket 1
ColabFold model
FPocket 0.635 · Pocket 7
P2Rank 0.032 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 7 / 4744 genomes with a hit
Normalized 0.001

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
2 224 Gene3D G3DSA:3.30.420.380 -
137 202 Gene3D G3DSA:3.30.420.370 -
1 373 NCBIfam TIGR01709 type II secretion system protein GspL
1 373 InterPro IPR007812 Type II secretion system protein GspL
2 139 SUPERFAMILY SSF53067 Actin-like ATPase domain
2 139 InterPro IPR043129 ATPase, nucleotide binding domain
2 226 Pfam PF05134 Type II secretion system (T2SS), protein L
2 226 InterPro IPR024230 GspL, cytoplasmic actin-ATPase-like domain
1 386 PIRSF PIRSF015761 Protein_L
1 386 InterPro IPR007812 Type II secretion system protein GspL
232 384 Pfam PF12693 GspL periplasmic domain
232 384 InterPro IPR025691 GspL periplasmic domain
296 387 Gene3D G3DSA:3.30.1360.100 General secretion pathway protein M, EpsM
143 224 SUPERFAMILY SSF53067 Actin-like ATPase domain
143 224 InterPro IPR043129 ATPase, nucleotide binding domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GNF4
AlphaFold — — full sequence — Viewing
ColabFold VK055_2416
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
11 0.733
4 0.334

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 0.64 0.001