KpATCC43816 Protein target profile

transporter, anaerobic C4-dicarboxylate uptake family protein

Accession: VK055_2936

Gene: AIK81522.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GL82
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Length 433
Pocket druggability (P2Rank · AlphaFold DB model) 0.939
Functional annotation 0 EC 4 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.94, FPocket 0.81). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.81. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 278 of 4744 screened genomes (5.9%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.94) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
5.9% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
37.086 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
93.74 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.939
Structure A0A0H3GL82
Pocket Pocket 1
Druggability (FPocket) 0.813
Structure A0A0H3GL82
Pocket Pocket 27
ColabFold model
P2Rank 0.888 · Pocket 1
FPocket 0.769 · Pocket 26
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 278 / 4744 genomes with a hit
Prevalence 5.9%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MFGAELVIVLLAIYLGARLGGIGIGFAGGLGVLVLTLIFQIKPGAIPFDVIEIIMAVIAAIAAMQVAGGMDYLVSLAERMLRRHPKYITFLAPLVTWFMTVLAGTGHTAFSTLPVITEVAKEQGIRPSRPLSIAVVASQIAITASPISAAVVFFAGILEPLGVSYLTLLAICIPVTLLAVMLTAIVCNFLGCELKDDPVYQERLAKGEVRLRGSQVFELQPHAKRSVLLFLIGIVAVMFYATAISDTVGLIKNPVLPRNEAIVVFMLTIATLISITCKIDTGEVLNASTFKSGMSACVCVLGVAWLGDTFVKAHISDIQAVAGDLLHNYPWLLAVVLFFAATLLYSQAATTKALMPAALLLGVSPLTAIASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKYVFNHAFLIPGVIAITLCVILGFIFGGIML

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

4
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0015740 The directed movement of a C4-dicarboxylate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. A C4-dicarboxylate is the anion of a dicarboxylic acid that contains four carbon atoms.
  • GO:0015556 Enables the transfer of C4-dicarboxylate from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

41 records
Show feature table
Start End DB Term Name
88 110 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
111 130 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
168 190 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
308 326 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
289 307 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
278 288 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
20 39 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 433 PIRSF PIRSF004539 Anaerob_C4-dicrbxlt_transp
1 433 InterPro IPR004668 Anaerobic c4-dicarboxylate membrane transporter
5 365 Pfam PF03605 Anaerobic c4-dicarboxylate membrane transporter
5 365 InterPro IPR004668 Anaerobic c4-dicarboxylate membrane transporter
359 381 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
385 403 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
163 190 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
227 249 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
46 67 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
250 260 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
357 384 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
329 346 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
404 428 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
346 356 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
40 45 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
284 306 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 433 PANTHER PTHR36106 ANAEROBIC C4-DICARBOXYLATE TRANSPORTER DCUB
5 433 NCBIfam TIGR00770 anaerobic C4-dicarboxylate transporter
5 433 InterPro IPR004668 Anaerobic c4-dicarboxylate membrane transporter
131 157 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
191 226 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
260 277 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
87 110 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
226 245 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
261 277 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
158 162 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
46 68 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
131 153 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
327 345 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
6 39 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
68 86 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
429 433 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
409 431 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.939
Likely same site as FPocket 1 1.9 Å 25 shared residues 96% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.861
Likely same site as FPocket 24 3.5 Å 15 shared residues 94% of smaller site
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Surrounding area
Pocket 3 P2Rank #3
0.093
Likely same site as FPocket 27 2.3 Å 9 shared residues 100% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.047
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Surrounding area
Pocket 5 P2Rank #5
0.046
Likely same site as FPocket 25 2.6 Å 7 shared residues 100% of smaller site
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #27
0.813
Likely same site as P2Rank 3 2.3 Å 9 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #25
0.553
Likely same site as P2Rank 5 2.6 Å 7 shared residues 100% of smaller site
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Surrounding area
Pocket 3 FPocket #1
0.487 Unusual size
Likely same site as P2Rank 1 1.9 Å 25 shared residues 96% of smaller site
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Surrounding area
Pocket 4 FPocket #24
0.445
Likely same site as P2Rank 2 3.5 Å 15 shared residues 94% of smaller site
Show in viewer
Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GL82
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_2936
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GL82