Protein profile

VK055_3069

manX

Genome: KpATCC43816

Gene: AIK81654.1 manX2 Structure source: Experimental + ColabFold UniProt P37081
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Amino acids 164
Annotations 6
Features 13
PDB binders 0
Druggability 0.153

Overview

Basic information about this protein and its source genome.

Accession
VK055_3069
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK81654.1 manX2
Status
annotated
Amino acids
164
Structure source
Experimental + ColabFold
EC
2.7.1.206
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0022871 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sorbose(out) = protein histidine + sorbose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
86.792
DEG E-value
5.14e-101
Localization
Cytoplasmic
ColabFold pLDDT
95.47

Selected Druggability evidence

PDB experimental structure

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.153
Structure 1NRZ
Pocket Pocket 1
P2Rank 0.042
Structure 1NRZ
Pocket Pocket 1
ColabFold model
FPocket 0.33 · Pocket 1
P2Rank 0.025 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 136 / 4744 genomes with a hit
Normalized 0.029

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0022871 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sorbose(out) = protein histidine + sorbose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

13 records
Show feature table
Start End DB Term Name
3 149 Pfam PF03830 PTS system sorbose subfamily IIB component
3 149 InterPro IPR004720 Phosphotransferase system, sorbose subfamily IIB component
3 153 CDD cd00001 PTS_IIB_man
3 153 InterPro IPR004720 Phosphotransferase system, sorbose subfamily IIB component
1 164 Gene3D G3DSA:3.40.35.10 Phosphotransferase system, sorbose subfamily IIB component
1 164 InterPro IPR036667 Phosphotransferase system, sorbose subfamily IIB component superfamily
1 164 ProSiteProfiles PS51101 PTS_EIIB type-4 domain profile.
1 164 InterPro IPR004720 Phosphotransferase system, sorbose subfamily IIB component
1 159 PANTHER PTHR33799 PTS PERMEASE-RELATED-RELATED
2 152 NCBIfam TIGR00854 mannose/fructose/sorbose PTS transporter subunit IIB
2 152 InterPro IPR018455 Phosphotransferase system, sorbose subfamily IIB component, subgroup
1 161 SUPERFAMILY SSF52728 PTS IIb component
1 161 InterPro IPR036667 Phosphotransferase system, sorbose subfamily IIB component superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 1NRZ
X-ray 1.75 Å A,B,C,D
100.0% 1-164
PDBe RCSB PDBj File
Viewing
ColabFold VK055_3069
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.499
55 0.208

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 28.29 0.924
2 16.09 0.77
3 7.48 0.393
4 7.4 0.388
5 7.32 0.382