Genome KpATCC43816

Protein target profile

sodium-dependent inorganic phosphate (Pi) transporter family protein

Accession: VK055_3074

Gene: AIK81659.1 pnaS 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GLT6
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Length 518
Pocket druggability (P2Rank) 0.605
Functional annotation 0 EC 3 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.60, FPocket 0.98). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.98. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 112 of 4744 screened genomes (2.4%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.60) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.4% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
86.87 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.605
Structure A0A0H3GLT6
Pocket Pocket 1
Druggability (FPocket) 0.98
Structure A0A0H3GLT6
Pocket Pocket 1
ColabFold model
P2Rank 0.092 · Pocket 1
FPocket 0.836 · Pocket 36
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 112 / 4744 genomes with a hit
Prevalence 2.4%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GLT6

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MRVYGARLRTVLSSSVEKKPLAFCAGLGVTALVQSSNATTMLVTSFVAQDLVGLMPALVMVLGADVGTALMARVLTFDLSWLSPLLIFIGVIFFLGRKQTRAGQLGRVGIGLGLILLALELIVQAVHPITQANGVQVIFASLTGDIMLDALIGAVFAIISYSSLAAVLLTATLTAAGAISFPVALCLVIGANLGSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHPLAAAMHKLPLAESELVIYFHVFYNLLRCIAMVPFAGPMAKLCQRLIRDEPELDNHLKPKHLDPSALDTPALALANAARETLRIGDAMEQMLESLHKVMHGEPRQEKELRRMADDINVLYTAIKLYLARMPKDELAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSVEGLKELDALYDLLLSNLQLAMSVFFSSDVPSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSTLHLALLGDMKRLNSLFCSVAYSVMEQPDEDNERDDY

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

3
  • GO:0044341 The directed movement of phosphate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore, by a mechanism dependent upon sodium ions.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005436 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: Na+(out) + phosphate(out) = Na+(in) + phosphate(in).

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

40 records
Show feature table
Start End DB Term Name
1 20 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
39 53 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
97 107 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
2 122 Pfam PF02690 Na+/Pi-cotransporter
2 122 InterPro IPR003841 Sodium-dependent phosphate transport protein
134 219 Pfam PF02690 Na+/Pi-cotransporter
134 219 InterPro IPR003841 Sodium-dependent phosphate transport protein
305 508 Gene3D G3DSA:1.20.58.220 Phosphate transport system protein phou homolog 2; domain 2
305 508 InterPro IPR038078 PhoU-like domain superfamily
130 149 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 283 NCBIfam TIGR00704 Na/Pi-cotransporter II-related protein
1 283 InterPro IPR004633 Na/Pi-cotransporter II-related/YqeW-like protein
150 173 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
108 129 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
42 495 NCBIfam TIGR01013 sodium-dependent inorganic phosphate transporter
42 495 InterPro IPR003841 Sodium-dependent phosphate transport protein
213 237 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
42 64 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
21 33 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
74 96 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
179 201 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
4 275 NCBIfam NF037997 Na/Pi symporter
3 77 PANTHER PTHR10010 SOLUTE CARRIER FAMILY 34 SODIUM PHOSPHATE , MEMBER 2-RELATED
3 77 InterPro IPR003841 Sodium-dependent phosphate transport protein
108 130 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
249 271 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
177 199 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
202 212 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
249 269 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
150 172 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
270 518 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
174 178 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
34 38 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
54 72 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 38 Phobius SIGNAL_PEPTIDE Signal peptide region
73 78 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
295 506 SUPERFAMILY SSF109755 PhoU-like
214 236 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
79 96 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
238 248 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.605
Likely same site as FPocket 20 0.9 Å 15 shared residues 94% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.094
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Surrounding area
Pocket 3 P2Rank #3
0.076
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Surrounding area
Pocket 4 P2Rank #4
0.063
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Surrounding area
Pocket 5 P2Rank #5
0.03
Likely same site as FPocket 1 2.0 Å 8 shared residues 100% of smaller site
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Surrounding area

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #1
0.98
Likely same site as P2Rank 5 2.0 Å 8 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #20
0.818
Likely same site as P2Rank 1 0.9 Å 15 shared residues 94% of smaller site
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Surrounding area
Pocket 3 FPocket #7
0.202
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GLT6
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_3074
ColabFold — — full sequence — Loaded