KpATCC43816 Protein target profile

aspT/YidE/YbjL antiporter duplication domain protein

Accession: VK055_3378

Gene: AIK81934.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3H4T9
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Length 553
Pocket druggability (P2Rank · AlphaFold DB model) 0.772
Functional annotation 0 EC 3 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.77, FPocket 0.82). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.82. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 118 of 4744 screened genomes (2.5%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.77) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.5% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
65.455 Higher values support similarity to known essential genes.
DEG E-value
0.0 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
88.47 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.772
Structure A0A0H3H4T9
Pocket Pocket 1
Druggability (FPocket) 0.817
Structure A0A0H3H4T9
Pocket Pocket 20
ColabFold model
P2Rank 0.791 · Pocket 1
FPocket 0.809 · Pocket 34
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 118 / 4744 genomes with a hit
Prevalence 2.5%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MSEIALTVSVLALVAVVGLWIGNVKIRGVGFGIGGVLFGGIIVGHFVDQAGVALSSPMLHFIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAILIVILGGLVTAVLHKLFNIPLPVVLGIFSGAVTNTPALGAGQQILRDLGVPFEVVDQMGMSYAMAYPFGICGILLTMWLVRLFFRINVEKEAQRFEESSGNGHAHLHTINVRVENPNLNQMAIQDVPMLNSDNIVCSRLKRGELLMVPAPGTLIQAGDLLHLVGRPEDLHNAQLVIGQEVATSLSTRGTDLKVERVVVTNEKVLGKKIRDLHVKQRYDVVISRLNRAGVELVASSSASLQFGDILNLVGRPEAIDAVAAELGNAQQKLQQVQMLPVFIGIGLGVLLGSIPLFIPGFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLAVVGLKSGGDFVATLTQGDGLSWIAYGIFITAIPLLTVGVLARMLAKMNYLTLCGMLAGSMTDPPALAFANNLHATSGAAALSYATVYPLVMFLRIITPQLLAVLFWGLS

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

3
  • GO:0006813 The directed movement of potassium ions (K+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0008324 Enables the transfer of cation from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

60 records
Show feature table
Start End DB Term Name
533 552 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
492 513 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
486 491 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
160 182 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
67 86 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
87 92 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
463 485 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
162 182 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
61 83 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
352 372 Coils Coil Coil
23 28 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
90 112 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
403 425 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
116 161 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
553 553 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
392 402 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
28 46 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
288 361 Gene3D G3DSA:3.30.70.1450 -
288 361 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
11 179 Pfam PF06826 Predicted Permease Membrane Region
11 179 InterPro IPR006512 YidE/YbjL duplication
374 546 Pfam PF06826 Predicted Permease Membrane Region
374 546 InterPro IPR006512 YidE/YbjL duplication
286 360 SUPERFAMILY SSF116726 TrkA C-terminal domain-like
286 360 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
6 22 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
459 463 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
29 47 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
48 66 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
437 459 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
426 436 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
279 361 ProSiteProfiles PS51202 RCK C-terminal domain profile.
279 361 InterPro IPR006037 Regulator of K+ conductance, C-terminal
464 485 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
15 551 PANTHER PTHR30445 UNCHARACTERIZED
4 21 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
205 272 Gene3D G3DSA:3.30.70.1450 -
205 272 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
437 458 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
93 115 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
371 393 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
403 425 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
203 271 SUPERFAMILY SSF116726 TrkA C-terminal domain-like
203 271 InterPro IPR036721 Regulator of K+ conductance, C-terminal domain superfamily
492 514 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 553 Hamap MF_01016 Putative transport protein YidE [yidE].
1 553 InterPro IPR023018 Transport protein YidE, putative
1 5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
514 532 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
292 360 Pfam PF02080 TrkA-C domain
292 360 InterPro IPR006037 Regulator of K+ conductance, C-terminal
183 370 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
192 276 ProSiteProfiles PS51202 RCK C-terminal domain profile.
192 276 InterPro IPR006037 Regulator of K+ conductance, C-terminal
371 391 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
16 166 NCBIfam TIGR01625 AspT/YidE/YbjL antiporter duplication domain
16 166 InterPro IPR006512 YidE/YbjL duplication
379 533 NCBIfam TIGR01625 AspT/YidE/YbjL antiporter duplication domain
379 533 InterPro IPR006512 YidE/YbjL duplication
529 551 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.772
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Surrounding area
Pocket 2 P2Rank #2
0.299
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Surrounding area
Pocket 3 P2Rank #3
0.254
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Surrounding area
Pocket 4 P2Rank #4
0.208
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Surrounding area
Pocket 5 P2Rank #5
0.189
Likely same site as FPocket 25 1.5 Å 16 shared residues 100% of smaller site
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #20
0.817
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Surrounding area
Pocket 2 FPocket #33
0.395 Unusual size
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Surrounding area
Pocket 3 FPocket #25
0.375 Unusual size
Likely same site as P2Rank 5 1.5 Å 16 shared residues 100% of smaller site
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Surrounding area
Pocket 4 FPocket #23
0.347
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3H4T9
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_3378
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3H4T9