KpATCC43816 Protein target profile

glutamine amidotransferase of anthranilate synthase/aminodeoxychorismate synthase family protein

Accession: VK055_3728

Gene: AIK82283.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GU55
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Length 187
Pocket druggability (P2Rank · AlphaFold DB model) 0.257
Direct ligand evidence 0 53 total records
Functional annotation 0 EC 6 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Structure available AlphaFold DB model + ColabFold model Ligand evidence via structure/bioactivity 3 structural and 0 bioactivity records.

Risks to review

Human similarity Top human hit identity: 33.929%. This target also has a predicted binding pocket (P2Rank 0.26) — check the ligand evidence before deprioritizing on off-target risk alone. Microbiome similarity 694 of 4744 screened genomes (14.6%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.26) — check the ligand evidence before deprioritizing on off-target risk alone. Weak predicted binding pocket FPocket selected-pocket score 0.11. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.26.

Evidence coverage

Core evidence streams are available for this target.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
33.929 Lower values reduce human off-target concern.
Human E-value
5.32e-11
Gut microbiome similarity
14.6% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
58.042 Higher values support similarity to known essential genes.
DEG E-value
4.44e-56 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
97.76 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.257
Structure A0A0H3GU55
Pocket Pocket 1
Druggability (FPocket) 0.107
Structure A0A0H3GU55
Pocket Pocket 2
ColabFold model
P2Rank 0.181 · Pocket 1
FPocket 0.249 · Pocket 5
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 694 / 4744 genomes with a hit
Prevalence 14.6%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MILLIDNYDSFTWNLYQYFCELGAEVLVRRNDALTLEEIAALAPEKIVISPGPCTPDEAGISLAVIRHYAGKTPLLGVCLGHQAIAQAFGATIVRAAQVMHGKTSLIEHNGEGVFQGLNNPLTVTRYHSLVIDPPTLPSEFNVTARSASGEIMGIRHREWDLEGVQFHPESILSEQGHQLLANFLKR

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Subcellular localization

Localization
Cytoplasmic

Gene Ontology (GO)

6
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0046820 Catalysis of the reaction: L-glutamine + chorismate = 4-amino-4-deoxychorismate + L-glutamate. It is composed of two enzymatic activities (which may be present on one or two polypeptides); the first is a glutaminase which yields ammonia from glutamine, releasing glutamate. The ammonia is used by the second activity which catalyzes the amination of chorismate to form 4-amino-4-deoxychorismate.
  • GO:0004049 Catalysis of the reaction: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
  • GO:0046656 The chemical reactions and pathways resulting in the formation of folic acid, pteroylglutamic acid.
  • GO:0000162 The chemical reactions and pathways resulting in the formation of L-tryptophan, the chiral amino acid 2-amino-3-(1H-indol-3-yl)propanoic acid; L-tryptophan is synthesized from chorismate via anthranilate.
  • GO:0046654 The chemical reactions and pathways resulting in the formation of tetrahydrofolate, 5,6,7,8-tetrahydrofolic acid, a folate derivative bearing additional hydrogens on the pterin group.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

24 records
Show feature table
Start End DB Term Name
2 185 CDD cd01743 GATase1_Anthranilate_Synthase
2 185 InterPro IPR006221 Anthranilate synthase/para-aminobenzoate synthase like domain
1 186 SUPERFAMILY SSF52317 Class I glutamine amidotransferase-like
1 186 InterPro IPR029062 Class I glutamine amidotransferase-like
2 186 PANTHER PTHR43418 MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED
47 56 PRINTS PR00097 Anthranilate synthase component II signature
119 131 PRINTS PR00097 Anthranilate synthase component II signature
164 177 PRINTS PR00097 Anthranilate synthase component II signature
2 16 PRINTS PR00097 Anthranilate synthase component II signature
99 107 PRINTS PR00097 Anthranilate synthase component II signature
74 85 PRINTS PR00097 Anthranilate synthase component II signature
1 187 FunFam G3DSA:3.40.50.880:FF:000003 Anthranilate synthase component II
3 185 Pfam PF00117 Glutamine amidotransferase class-I
3 185 InterPro IPR017926 Glutamine amidotransferase
1 187 NCBIfam TIGR00566 glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
1 187 InterPro IPR006221 Anthranilate synthase/para-aminobenzoate synthase like domain
1 187 Gene3D G3DSA:3.40.50.880 -
1 187 InterPro IPR029062 Class I glutamine amidotransferase-like
1 187 ProSiteProfiles PS51273 Glutamine amidotransferase type 1 domain profile.
74 85 PRINTS PR00096 Glutamine amidotransferase superfamily signature
47 56 PRINTS PR00096 Glutamine amidotransferase superfamily signature
164 177 PRINTS PR00096 Glutamine amidotransferase superfamily signature
74 90 PRINTS PR00099 Carbamoyl-phosphate synthase protein GATase domain signature
44 58 PRINTS PR00099 Carbamoyl-phosphate synthase protein GATase domain signature

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

Download VMD script Full viewer

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.257
Show in viewer
Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GU55
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_3728
ColabFold — — full sequence — Loaded

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records
Chemistry signal

Structural ligand evidence is available for this target.

Direct evidence 0 same-protein records
Transferred evidence 3 records from similar proteins
Structural ligands 3 0 loaded crystals
Measured bioactivity 0 direct and transferred ChEMBL records
Proposed compounds 50 similarity-based ZINC candidates
Best available ligand signal
IMP PDB via homolog 348.2 Da · LogP -2.15 · TPSA 180.0 Open detail RCSB PDB
ORN PDB via homolog Detail RCSB PDB
POP PDB via homolog Detail RCSB PDB
ZINC14951284 ZINC proposed compound · Tanimoto 1.000 Detail ZINC
ZINC1532551 ZINC proposed compound · Tanimoto 1.000 Detail ZINC

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
IMP RCSB PDB
1CE8
P0A6F1 348.2 Da LogP -2.15 TPSA 180.0 ✓ Ro5 ✓ Clean c1nc2c(n1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O…
ORN RCSB PDB
1CE8
P0A6F1 132.2 Da LogP -0.86 TPSA 89.3 ✓ Ro5 ✓ Clean C(C[C@@H](C(=O)O)N)CN
POP RCSB PDB
1GPM
P04079 176.0 Da LogP -2.08 TPSA 129.9 ✓ Ro5 ✓ Clean O[P@@](=O)([O-])O[P@@](=O)(O)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GU55