KpATCC43816 Protein target profile

mtr tryptophan ArAAP transporter

Accession: VK055_3910

Gene: mtr AIK82458.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3H3G8
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Length 414
Pocket druggability (P2Rank · AlphaFold DB model) 0.954
Functional annotation 0 EC 4 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Second pocket predictor agrees P2Rank predicted binding-site probability 0.95. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 123 of 4744 screened genomes (2.6%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.95) — check the ligand evidence before deprioritizing on off-target risk alone. Best pocket may be a modeling artifact FPocket selected-pocket score 0.95, but this pocket's geometry is unusually large/diffuse compared to this protein's other predicted pockets — may reflect a low-confidence or disordered model region rather than a real cavity.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.6% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
51.843 Higher values support similarity to known essential genes.
DEG E-value
1.41e-142 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
88.88 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.954
Structure A0A0H3H3G8
Pocket Pocket 1
Druggability (FPocket) 0.95
Structure A0A0H3H3G8
Pocket Pocket 21
ColabFold model
P2Rank 0.946 · Pocket 1
FPocket 0.737 · Pocket 5
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 123 / 4744 genomes with a hit
Prevalence 2.6%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MATLTTTATRPSLFGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSLAALVFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNLVNGVSIAFVLYILTYAYISASGSILHHTFSELSLKVPARAAGFGFALLVAFIVWMSTKAVSRMTAIVLGAKVITFFLTFGSLLGHVEPTTLFNVAEKNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPRTIIRCLTYGTLLALGLYVVWLLVTMGNIPRPQFIDIAQKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRFKTALLTFIPPMIGGLVKPDGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPQFRVWGGTPMIVLILLFGLGNAVVHILSSVNLLPVYQ

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

4
  • GO:0003333 The process in which an amino acid is transported across a membrane.
  • GO:0015173 Enables the transfer of aromatic amino acids from one side of a membrane to the other. Aromatic amino acids have an aromatic ring.
  • GO:0015801 The directed movement of aromatic amino acids, amino acids with aromatic ring, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

62 records
Show feature table
Start End DB Term Name
345 367 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
40 65 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
321 339 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
310 320 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
66 85 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
344 366 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 413 Gene3D G3DSA:1.20.1740.10 Amino acid/polyamine transporter I
190 207 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
368 378 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
284 309 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
171 189 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
15 395 NCBIfam TIGR00837 aromatic amino acid transporter
15 395 InterPro IPR013059 Tryptophan/tyrosine transporter
152 170 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
39 61 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
87 109 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
25 41 ProSitePatterns PS00594 Aromatic amino acids permeases signature.
25 41 InterPro IPR013061 Tryptophan/tryrosine permease, conserved site
10 413 FunFam G3DSA:1.20.1740.10:FF:000019 Tryptophan permease TnaB
155 175 PRINTS PR00166 Aromatic amino acid permease signature
155 175 InterPro IPR013059 Tryptophan/tyrosine transporter
45 64 PRINTS PR00166 Aromatic amino acid permease signature
45 64 InterPro IPR013059 Tryptophan/tyrosine transporter
232 251 PRINTS PR00166 Aromatic amino acid permease signature
232 251 InterPro IPR013059 Tryptophan/tyrosine transporter
293 312 PRINTS PR00166 Aromatic amino acid permease signature
293 312 InterPro IPR013059 Tryptophan/tyrosine transporter
348 367 PRINTS PR00166 Aromatic amino acid permease signature
348 367 InterPro IPR013059 Tryptophan/tyrosine transporter
18 41 PRINTS PR00166 Aromatic amino acid permease signature
18 41 InterPro IPR013059 Tryptophan/tyrosine transporter
90 110 PRINTS PR00166 Aromatic amino acid permease signature
90 110 InterPro IPR013059 Tryptophan/tyrosine transporter
193 215 PRINTS PR00166 Aromatic amino acid permease signature
193 215 InterPro IPR013059 Tryptophan/tyrosine transporter
327 345 PRINTS PR00166 Aromatic amino acid permease signature
327 345 InterPro IPR013059 Tryptophan/tyrosine transporter
7 414 PANTHER PTHR46997 LOW AFFINITY TRYPTOPHAN PERMEASE-RELATED
7 414 InterPro IPR013059 Tryptophan/tyrosine transporter
35 39 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
86 107 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
405 414 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
208 227 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
225 247 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
284 306 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
129 146 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
247 283 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
153 175 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
379 401 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
146 151 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
10 402 Pfam PF03222 Tryptophan/tyrosine permease family
10 402 InterPro IPR018227 Amino acid/polyamine transporter 2
108 126 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
127 145 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 34 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
319 339 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
379 404 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
340 344 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
190 212 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
228 246 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.954
Likely same site as FPocket 21 1.1 Å 37 shared residues 90% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.636
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Surrounding area
Pocket 3 P2Rank #3
0.26
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Surrounding area
Pocket 4 P2Rank #4
0.126
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Surrounding area
Pocket 5 P2Rank #5
0.108
Likely same site as FPocket 13 2.3 Å 10 shared residues 100% of smaller site
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #21
0.95 Unusual size
Likely same site as P2Rank 1 1.1 Å 37 shared residues 90% of smaller site
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Surrounding area
Pocket 2 FPocket #13
0.759
Likely same site as P2Rank 5 2.3 Å 10 shared residues 100% of smaller site
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Surrounding area
Pocket 3 FPocket #2
0.53
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3H3G8
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_3910
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3H3G8