KpATCC43816 Protein target profile

nupG nucleoside MFS transporter

Accession: VK055_4079

Gene: AIK82625.1 nupG 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GT64
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Length 417
Pocket druggability (P2Rank · AlphaFold DB model) 0.899
Functional annotation 0 EC 9 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Moderate predicted binding pocket FPocket selected-pocket score 0.59. This supports review but is not experimental validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.90. Review the complete evidence below

Risks to review

Microbiome similarity 151 of 4744 screened genomes (3.2%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.90) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
3.2% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
43.405 Higher values support similarity to known essential genes.
DEG E-value
8.74e-112 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
92.35 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.899
Structure A0A0H3GT64
Pocket Pocket 1
Druggability (FPocket) 0.589
Structure A0A0H3GT64
Pocket Pocket 20
ColabFold model
P2Rank 0.899 · Pocket 1
FPocket 0.688 · Pocket 11
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 151 / 4744 genomes with a hit
Prevalence 3.2%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGAAIGAVYSSLGIAAVLMPTLLGIVADKWISAKWVYAICHLVGALTLYLAAQVTTPGEMFLVILLNSLAYMPTLGLINTISYYRLQSAGLDIVTDFPPIRIWGTIGFILAMWGVSFSGFELSHMQLYIGATLSVLLTLFTLTLPHIPVANAQRNQSWTEMLGLNAFALFKNKRMAIFFIFSMMLGAELQITNMFGNTFLHSFDKDPLFAGSFIVEHASVLMSISQISETLFILTIPFFLSRYGIKNVMLISIVAWMLRFGLFAFGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKEVRPEIRASAQGMFLMMTNGFGCILGGMVSGKVVEHFTVEGITDWQSVWLIFAGYSLVLAFAFVALFKYKHVRQPTAAQQSV

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

9
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005337 Enables the transfer of a nucleoside, a nucleobase linked to either beta-D-ribofuranose (ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleotide) from one side of a membrane to the other.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:1901642 The directed movement of nucleoside across a membrane.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015858 The directed movement of a nucleoside, a nucleobase linked to either beta-D-ribofuranose (ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleotide), into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0015212 Enables the transfer of cytidine, cytosine riboside, from one side of a membrane to the other.
  • GO:0015506 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: nucleoside(out) + H+(out) = nucleoside(in) + H+(in).
  • GO:0015213 Enables the transfer of uridine, uracil riboside, from one side of a membrane to the other.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

55 records
Show feature table
Start End DB Term Name
137 155 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
37 58 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
211 235 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
161 179 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
156 160 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
95 114 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
253 275 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 403 CDD cd06177 MFS_NHS
306 327 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
95 116 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
117 136 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
311 333 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
195 404 FunFam G3DSA:1.20.1250.20:FF:000015 Nucleoside permease NupG
162 184 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
90 94 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
195 403 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
195 403 InterPro IPR036259 MFS transporter superfamily
5 189 FunFam G3DSA:1.20.1250.20:FF:000012 Nucleoside permease NupG
135 152 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
39 61 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
348 369 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
255 275 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 300 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
2 412 PANTHER PTHR23522 BLL5896 PROTEIN
1 414 NCBIfam TIGR00889 nucleoside permease
1 414 InterPro IPR004740 Nucleoside:H+ symporter
370 380 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 406 Pfam PF03825 Nucleoside H+ symporter
1 406 InterPro IPR004740 Nucleoside:H+ symporter
301 305 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
68 90 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
59 69 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
32 36 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
403 417 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
9 406 SUPERFAMILY SSF103473 MFS general substrate transporter
9 406 InterPro IPR036259 MFS transporter superfamily
209 417 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
209 417 InterPro IPR020846 Major facilitator superfamily domain
328 347 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 417 Hamap MF_02049 Nucleoside permease NupG [nupG].
1 417 InterPro IPR033667 Nucleoside permease NupG
380 402 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
346 365 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
211 233 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
236 254 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
381 402 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 301 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
276 281 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
180 210 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
70 89 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
5 185 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
5 185 InterPro IPR036259 MFS transporter superfamily

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.899
Likely same site as FPocket 6 1.5 Å 24 shared residues 92% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.332
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Surrounding area
Pocket 3 P2Rank #3
0.273
Likely same site as FPocket 20 1.0 Å 10 shared residues 100% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.252
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Surrounding area
Pocket 5 P2Rank #5
0.136
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #20
0.589
Likely same site as P2Rank 3 1.0 Å 10 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #6
0.291
Likely same site as P2Rank 1 1.5 Å 24 shared residues 92% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GT64
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_4079
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GT64