KpATCC43816 Protein target profile

type VII secretion system (T7SS), usher family protein

Accession: VK055_4230

Gene: AIK82775.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GY15
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Length 870
Pocket druggability (P2Rank · AlphaFold DB model) 0.638
Functional annotation 0 EC 5 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Second pocket predictor agrees P2Rank predicted binding-site probability 0.64. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 37 of 4744 screened genomes (0.8%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.64) — check the ligand evidence before deprioritizing on off-target risk alone. Weak predicted binding pocket FPocket selected-pocket score 0.35.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
0.8% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
59.212 Higher values support similarity to known essential genes.
DEG E-value
0.0 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
87.46 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.638
Structure A0A0H3GY15
Pocket Pocket 1
Druggability (FPocket) 0.349
Structure A0A0H3GY15
Pocket Pocket 8
ColabFold model
P2Rank 0.61 · Pocket 1
FPocket 0.699 · Pocket 74
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 37 / 4744 genomes with a hit
Prevalence 0.8%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MSHRKYGLDHLGCRTARRLVSPALALWLCSQPFAARADLYFNPRFLADDPAAVADLSGFEKGQEVPPGTYRVDIYLNNGFMTTRDVTFQADAQGHGLSPCLTRGQLASMGVDTGRVPGMATLDSTACVPLTTLISEATTRFDVGQQRLYLTVPQAFMGNHARGYIPPELWDNGITAGLINYNFTGNNAHNTTGGSSRYAYLNLQSGLNIGAWRLRDNSTWSYSSGGSTSSNENRWQHVNSWLERDITPLRSRLTLGDSYTNGDVFDGINFRGAQLASDDNMLPDSQKGFAPVIHGIARGTAQVSIRQNGYEIYQSTVPPGPFTIDDLYAAGNGGDLQVTIKEADGSRQVFSVPWSTVPVLQREGHTRFALTAGEYRSGNSQQETPDFFQGTAMHGLPAGWTLYGGTQLADRYRAFNLGVGKNMGYFGALSLDITQANATLADDSEHQGQSVRFLYNKSLDETGTNLQLVGYRYSTRGYYNFADTTYRRMSGYSVETQDGVIQVKPKFTDYYNLAYSKRGKVQLSVTQQLGRTATLYLSGSHQTYWGTDDADEQLQAGLNAAVDDINWSLSYSLTKNAWQQGRDQMLAININIPFSHWLRSDSRSVWRHASASYSLSHDLNGRMTNLAGLYGTLLEDNNLSYSVQTGYAGGGNGDNGSTGYTALNYRGGYGNANVGYSRSDGFKQLYYGVSGGVLAHANGITLSQPLNDTVVLVKAPGAGGVKVENQTGVRTDWRGYAVLPYATEYRENRIALDTNTLADNVDLDDAVVSVVPTHGAIVRANFNAQVGMKILMTLTHRGKPVPFGALATGDSNQSGSIVADNGQVYLSGMPLAGKVRVKWGDGPDAQCVADYRLPPESQQQALSQLSVACR

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
OuterMembrane

Gene Ontology (GO)

5
  • GO:0015473 A porin that acts in the assembly of fimbria together with fimbrial chaperone.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005515 Binding to a protein.
  • GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles.
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

27 records
Show feature table
Start End DB Term Name
19 29 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
200 782 Pfam PF00577 Outer membrane usher protein
200 782 InterPro IPR000015 Outer membrane usher protein
1 37 Phobius SIGNAL_PEPTIDE Signal peptide region
697 787 FunFam G3DSA:2.60.40.2610:FF:000001 Outer membrane fimbrial usher protein
275 364 FunFam G3DSA:2.60.40.3110:FF:000001 Putative fimbrial outer membrane usher
38 870 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
275 364 Gene3D G3DSA:2.60.40.3110 -
40 184 Pfam PF13954 PapC N-terminal domain
40 184 InterPro IPR025885 PapC, N-terminal domain
788 870 Gene3D G3DSA:2.60.40.2070 -
788 870 InterPro IPR043142 PapC-like, C-terminal domain superfamily
1 18 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
38 162 Gene3D G3DSA:3.10.20.410 -
38 162 InterPro IPR037224 PapC, N-terminal domain superfamily
697 787 Gene3D G3DSA:2.60.40.2610 Outer membrane usher protein FimD, plug domain
697 787 InterPro IPR042186 Outer membrane usher protein FimD, plug domain
63 176 SUPERFAMILY SSF141729 FimD N-terminal domain-like
63 176 InterPro IPR037224 PapC, N-terminal domain superfamily
788 870 FunFam G3DSA:2.60.40.2070:FF:000001 Fimbrial outer membrane usher protein
317 327 ProSitePatterns PS01151 Fimbrial biogenesis outer membrane usher protein signature.
317 327 InterPro IPR018030 Fimbrial membrane usher, conserved site
30 37 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
18 869 PANTHER PTHR30451 OUTER MEMBRANE USHER PROTEIN
18 869 InterPro IPR000015 Outer membrane usher protein
791 855 Pfam PF13953 PapC C-terminal domain
791 855 InterPro IPR025949 PapC-like, C-terminal domain

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.638
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Surrounding area
Pocket 2 P2Rank #2
0.286
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Surrounding area
Pocket 3 P2Rank #3
0.126
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Surrounding area
Pocket 4 P2Rank #4
0.075
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Surrounding area
Pocket 5 P2Rank #5
0.072
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #8
0.349
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Surrounding area
Pocket 2 FPocket #73
0.303
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Surrounding area
Pocket 3 FPocket #67
0.204
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GY15
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_4230
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GY15