Protein profile

VK055_4231

gram-negative pili assembly chaperone, C-terminal domain protein

Genome: KpATCC43816

Gene: AIK82776.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GST7
Export view
Amino acids 219
Annotations 4
Features 34
PDB binders 3
Druggability 0.907

Overview

Basic information about this protein and its source genome.

Accession
VK055_4231
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK82776.1
Status
annotated
Amino acids
219
Structure source
AlphaFold + ColabFold
GO
GO:0043711 A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a pilus, a short filamentous structure on a bacterial cell, flagella-like in structure and generally present in many copies. GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. GO:0061077 OBSOLETE. The process of inhibiting aggregation and assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure that is dependent on interaction with a chaperone.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
82.648
DEG E-value
5.52e-139
Localization
Periplasmic
ColabFold pLDDT
92.09

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.907
Structure A0A0H3GST7
Pocket Pocket 2
P2Rank 0.037
Structure A0A0H3GST7
Pocket Pocket 1
ColabFold model
FPocket 0.519 · Pocket 2
P2Rank 0.01 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 55 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0043711 A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a pilus, a short filamentous structure on a bacterial cell, flagella-like in structure and generally present in many copies.
  • GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0061077 OBSOLETE. The process of inhibiting aggregation and assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure that is dependent on interaction with a chaperone.

Sequence Features

Domain/signature hits from InterPro and related databases.

34 records
Show feature table
Start End DB Term Name
157 211 Pfam PF02753 Pili assembly chaperone PapD, C-terminal domain
157 211 InterPro IPR016148 Pili assembly chaperone, C-terminal
16 132 FunFam G3DSA:2.60.40.10:FF:000458 Molecular chaperone FimC
136 219 Gene3D G3DSA:2.60.40.10 Immunoglobulins
136 219 InterPro IPR013783 Immunoglobulin-like fold
2 12 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 19 SignalP_EUK SignalP-noTM SignalP-noTM
20 219 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
116 131 PRINTS PR00969 Pili chaperone signature
116 131 InterPro IPR001829 Pili assembly chaperone, bacterial
61 82 PRINTS PR00969 Pili chaperone signature
61 82 InterPro IPR001829 Pili assembly chaperone, bacterial
16 25 PRINTS PR00969 Pili chaperone signature
16 25 InterPro IPR001829 Pili assembly chaperone, bacterial
90 107 PRINTS PR00969 Pili chaperone signature
90 107 InterPro IPR001829 Pili assembly chaperone, bacterial
151 166 PRINTS PR00969 Pili chaperone signature
151 166 InterPro IPR001829 Pili assembly chaperone, bacterial
89 106 ProSitePatterns PS00635 Gram-negative pili assembly chaperone signature.
89 106 InterPro IPR018046 Pili assembly chaperone, conserved site
15 134 Pfam PF00345 Pili and flagellar-assembly chaperone, PapD N-terminal domain
15 134 InterPro IPR016147 Pili assembly chaperone, N-terminal
1 19 Phobius SIGNAL_PEPTIDE Signal peptide region
129 213 SUPERFAMILY SSF49584 Periplasmic chaperone C-domain
129 213 InterPro IPR036316 Pili assembly chaperone, C-terminal domain superfamily
1 14 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
15 134 SUPERFAMILY SSF49354 PapD-like
15 134 InterPro IPR008962 PapD-like superfamily
13 19 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
16 131 Gene3D G3DSA:2.60.40.10 Immunoglobulins
16 131 InterPro IPR013783 Immunoglobulin-like fold
1 1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
2 215 PANTHER PTHR30251 PILUS ASSEMBLY CHAPERONE
1 19 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GST7
AlphaFold — — full sequence — Viewing
ColabFold VK055_4231
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.907
1 0.307

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.62 0.026

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

3 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
EC2
2XG5
P15319 453.6 Da LogP 5.94 TPSA 59.3 1 viol. ✓ Clean c1ccc(cc1)[C@@H]2[C@H](N3C(=O)C=C(C(=C3S2)C4CC4…
EC5
2XG5
P15319 471.6 Da LogP 5.90 TPSA 79.5 1 viol. ✓ Clean c1ccc(cc1)C[C@@H](C(=O)O)N2C(=O)C=C(C(=C2SO)C3C…
XC2
2XG4
P15319 476.6 Da LogP 4.03 TPSA 71.8 ✓ Ro5 ✓ Clean c1ccc2c(c1)cccc2CC3=C(C(=O)N4[C@@H](CSC4=C3C5CC…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.