KpATCC43816 Protein target profile

H+ symporter family protein

Accession: VK055_4591

Gene: AIK83125.1 3D evidence: AlphaFold DB model + ColabFold model Metabolism Not in network UniProt A0A0H3GRV4
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Length 432
Pocket druggability (P2Rank · AlphaFold DB model) 0.824
Functional annotation 0 EC 5 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.82, FPocket 0.93). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.93. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 118 of 4744 screened genomes (2.5%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.82) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.5% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
50.12 Higher values support similarity to known essential genes.
DEG E-value
3.81e-149 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
87.74 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.824
Structure A0A0H3GRV4
Pocket Pocket 1
Druggability (FPocket) 0.933
Structure A0A0H3GRV4
Pocket Pocket 32
ColabFold model
P2Rank 0.683 · Pocket 1
FPocket 0.626 · Pocket 8
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 118 / 4744 genomes with a hit
Prevalence 2.5%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

This protein is not associated with the imported metabolic network for this genome.

Browse the genome's metabolic network

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MTESSITERGAPELADTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPAGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADRRGRKTSMLISVCMMCFGSLVIACLPGYAVIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGKKGFYASFQYVTLIGGQLLAVLVVVALQQVLSDEDLHAWGWRIPFALGAVLAIVALWLRRQLDETSKQETRALKEAGSFKGLWRNRRAFAMVLGFTAAGSLTFYTFTTYMQKYLVNTAGMTASTASVIMTAALFVYMLVQPLFGAFSDKVGRRTSMLCFGVLATLFTVPILSALQKVSSPYAAFGLVICALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSAGIEHAFYWYVTVMAAIAFLVSLMLHRKGKGLRL

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

5
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015293 Enables the active transport of a solute across a membrane by a mechanism whereby two or more species are transported together in the same direction in a tightly coupled process not directly linked to a form of energy other than chemiosmotic energy.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

52 records
Show feature table
Start End DB Term Name
195 214 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
160 182 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 427 PANTHER PTHR43528 ALPHA-KETOGLUTARATE PERMEASE
312 331 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
140 159 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 37 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
215 243 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
133 158 ProSitePatterns PS00217 Sugar transport proteins signature 2.
133 158 InterPro IPR005829 Sugar transporter, conserved site
337 360 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
192 214 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
361 371 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
19 432 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
19 432 InterPro IPR036259 MFS transporter superfamily
62 84 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
19 430 SUPERFAMILY SSF103473 MFS general substrate transporter
19 430 InterPro IPR036259 MFS transporter superfamily
24 427 CDD cd17367 MFS_KgtP
19 432 FunFam G3DSA:1.20.1250.20:FF:000095 Alpha-ketoglutarate permease
85 95 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
404 423 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
160 183 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
117 121 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
372 393 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
264 268 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
281 303 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
337 359 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
394 404 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
244 263 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
97 119 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
269 292 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
184 194 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
405 423 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
244 266 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
23 428 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
23 428 InterPro IPR020846 Major facilitator superfamily domain
57 61 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
122 139 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
372 394 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
38 56 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
62 84 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
310 327 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
123 140 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
24 420 Pfam PF00083 Sugar (and other) transporter
24 420 InterPro IPR005828 Major facilitator, sugar transporter-like
424 432 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
293 311 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
332 336 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
96 116 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
30 418 NCBIfam TIGR00883 metabolite/H+ symporter
30 418 InterPro IPR004736 MFS transporter, metabolite:H symporter

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.824
Likely same site as FPocket 16 5.0 Å 22 shared residues 76% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.408
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Surrounding area
Pocket 3 P2Rank #3
0.031
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Surrounding area
Pocket 4 P2Rank #4
0.017
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Surrounding area
Pocket 5 P2Rank #5
0.011
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #32
0.933
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Surrounding area
Pocket 2 FPocket #17
0.911
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Surrounding area
Pocket 3 FPocket #11
0.833
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Surrounding area
Pocket 4 FPocket #16
0.552 Unusual size
Likely same site as P2Rank 1 5.0 Å 22 shared residues 76% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GRV4
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_4591
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GRV4