Protein profile

VK055_5077

thiF family protein

Genome: KpATCC43816

Gene: AIK83603.1 Structure source: AlphaFold + ColabFold UniProt A4GZH6
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Amino acids 464
Annotations 7
Features 10
PDB binders 0
Druggability 0.547

Overview

Basic information about this protein and its source genome.

Accession
VK055_5077
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK83603.1
Status
annotated
Amino acids
464
Structure source
AlphaFold + ColabFold
GO
GO:0008641 Catalysis of the activation of small proteins, such as ubiquitin or ubiquitin-like proteins, through the formation of an ATP-dependent high-energy thiolester bond. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0046872 Binding to a metal ion. GO:0008237 Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. GO:0016779 Catalysis of the transfer of a nucleotidyl group from one compound (donor) to another (acceptor). GO:0004792 Catalysis of the reaction: thiosulfate + hydrogen cyanide = thiocyanate + sulfite + 2 H+.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
92.45

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.547
Structure A4GZH6
Pocket Pocket 5
P2Rank 0.829
Structure A4GZH6
Pocket Pocket 1
ColabFold model
FPocket 0.171 · Pocket 24
P2Rank 0.863 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 2 / 4744 genomes with a hit
Normalized 0

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0008641 Catalysis of the activation of small proteins, such as ubiquitin or ubiquitin-like proteins, through the formation of an ATP-dependent high-energy thiolester bond.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0046872 Binding to a metal ion.
  • GO:0008237 Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
  • GO:0016779 Catalysis of the transfer of a nucleotidyl group from one compound (donor) to another (acceptor).
  • GO:0004792 Catalysis of the reaction: thiosulfate + hydrogen cyanide = thiocyanate + sulfite + 2 H+.
  • GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.

Sequence Features

Domain/signature hits from InterPro and related databases.

10 records
Show feature table
Start End DB Term Name
197 317 CDD cd01483 E1_enzyme_family
184 429 Pfam PF00899 ThiF family
184 429 InterPro IPR000594 THIF-type NAD/FAD binding fold
191 334 PANTHER PTHR10953 UBIQUITIN-ACTIVATING ENZYME E1
191 334 InterPro IPR045886 ThiF/MoeB/HesA family
19 139 Pfam PF14464 Prokaryotic homologs of the JAB domain
19 139 InterPro IPR028090 JAB domain, prokaryotic
192 447 Gene3D G3DSA:3.40.50.720 -
191 406 SUPERFAMILY SSF69572 Activating enzymes of the ubiquitin-like proteins
191 406 InterPro IPR035985 Ubiquitin-activating enzyme

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A4GZH6
AlphaFold — — full sequence — Viewing
ColabFold VK055_5077
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
23 0.542
2 0.169
1 0.052
32 0.014

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.58 0.829
2 6.16 0.255
3 3.83 0.12
4 3.19 0.087
5 3.03 0.08