Protein profile

KP13_00068

PTS system alpha-glucoside-specific EIICB component

Genome: KpKP13

Gene: AHE41991.1 aglA Structure source: AlphaFold + ColabFold UniProt A0A0H3GZ00
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Amino acids 583
Annotations 6
Features 58
PDB binders 0
Druggability 0.742

Overview

Basic information about this protein and its source genome.

Accession
KP13_00068
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE41991.1 aglA
Status
annotated
Amino acids
583
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
82.74

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.742
Structure A0A0H3GZ00
Pocket Pocket 8
P2Rank 0.977
Structure A0A0H3GZ00
Pocket Pocket 1
ColabFold model
FPocket 0.724 · Pocket 11
P2Rank 0.971 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 157 / 4744 genomes with a hit
Normalized 0.033

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

58 records
Show feature table
Start End DB Term Name
266 276 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 53 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
242 264 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
42 463 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
42 463 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
348 366 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
299 317 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
277 298 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
205 227 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
44 569 PANTHER PTHR30009 CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT
54 75 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
494 567 SUPERFAMILY SSF55604 Glucose permease domain IIB
494 567 InterPro IPR036878 Glucose permease domain IIB
492 570 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
492 570 InterPro IPR036878 Glucose permease domain IIB
367 371 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
172 195 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
506 523 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
506 523 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
106 123 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
130 152 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
276 298 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
103 401 NCBIfam TIGR00852 maltose/glucose-specific PTS transporter subunit IIC
103 401 InterPro IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component
45 567 NCBIfam TIGR02005 alpha-glucoside-specific PTS transporter subunit IIBC
45 567 InterPro IPR010975 Phosphotransferase system, alpha-glucoside-specific IIBC component
493 567 CDD cd00212 PTS_IIB_glc
493 567 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
76 105 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
415 425 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
389 394 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
372 388 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
134 156 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
342 347 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
387 409 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
497 526 Pfam PF00367 phosphotransferase system, EIIB
497 526 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
235 245 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
124 129 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
452 553 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
452 553 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
196 206 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
207 234 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
395 414 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
448 583 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
491 573 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
491 573 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
321 343 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
246 265 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
153 171 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
51 73 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
171 193 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
350 372 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
318 341 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
426 447 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
430 452 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
53 389 Pfam PF02378 Phosphotransferase system, EIIC
53 389 InterPro IPR003352 Phosphotransferase system, EIIC

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GZ00
AlphaFold — — full sequence — Viewing
ColabFold KP13_00068
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
8 0.742
1 0.591
12 0.202

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 40.07 0.963
2 7.72 0.409
3 4.42 0.186
4 2.27 0.057
5 2.18 0.052