Protein profile

KP13_00084

Sensor protein uhpB

Genome: KpKP13

Gene: uhpB AHE42009.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GV46
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Amino acids 500
Annotations 5
Features 42
PDB binders 1
Druggability 0.775

Overview

Basic information about this protein and its source genome.

Accession
KP13_00084
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
uhpB AHE42009.1
Status
annotated
Amino acids
500
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0046983 The formation of a protein dimer, a macromolecular structure consists of two noncovalently associated identical or nonidentical subunits. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.86

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.775
Structure A0A0H3GV46
Pocket Pocket 19
P2Rank 0.296
Structure A0A0H3GV46
Pocket Pocket 1
ColabFold model
FPocket 0.827 · Pocket 26
P2Rank 0.253 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 58 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0046983 The formation of a protein dimer, a macromolecular structure consists of two noncovalently associated identical or nonidentical subunits.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

42 records
Show feature table
Start End DB Term Name
101 111 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
273 300 Coils Coil Coil
276 362 Gene3D G3DSA:1.20.5.1930 -
231 249 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
255 273 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
183 201 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
133 137 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
274 500 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
138 162 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
163 182 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
140 162 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
202 206 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
220 242 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
61 79 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
6 26 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
250 254 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
384 497 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
384 497 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
225 230 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
186 205 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
311 499 ProSiteProfiles PS50109 Histidine kinase domain profile.
311 499 InterPro IPR005467 Histidine kinase domain
112 132 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
112 130 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
414 496 CDD cd16917 HATPase_UhpB-NarQ-NarX-like
78 100 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
80 100 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
207 224 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
14 275 Pfam PF05231 MASE1
14 275 InterPro IPR007895 MASE1
38 60 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
305 370 Pfam PF07730 Histidine kinase
305 370 InterPro IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain
255 274 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
27 37 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
375 497 Gene3D G3DSA:3.30.565.10 -
375 497 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
95 498 PANTHER PTHR24421 NITRATE/NITRITE SENSOR PROTEIN NARX-RELATED
409 499 SMART SM00387 HKATPase_4
409 499 InterPro IPR003594 Histidine kinase/HSP90-like ATPase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GV46
AlphaFold — — full sequence — Viewing
ColabFold KP13_00084
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
19 0.775
37 0.729
2 0.523

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 5.73 0.277
2 5.19 0.24
3 2.91 0.093
4 2.26 0.056
5 1.84 0.036

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

51 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ACP
3EHJ
O34757 505.2 Da LogP -1.52 TPSA 269.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.