Protein profile

KP13_00090

Purine ribonucleoside efflux pump nepI

Genome: KpKP13

Gene: AHE42015.1 nepI Structure source: AlphaFold + ColabFold UniProt A0A0H3H4R6
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Amino acids 397
Annotations 7
Features 49
PDB binders 0
Druggability 0.716

Overview

Basic information about this protein and its source genome.

Accession
KP13_00090
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42015.1 nepI
Status
annotated
Amino acids
397
Structure source
AlphaFold + ColabFold
GO
GO:0015211 Enables the transfer of a purine nucleoside, a purine base covalently bonded to a ribose or deoxyribose sugar, from one side of a membrane to the other. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0015860 The process in which a purine nucleoside is transported across a membrane. A purine nucleoside is a purine base covalently bonded to a ribose or deoxyribose sugar. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
92.21

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.716
Structure A0A0H3H4R6
Pocket Pocket 24
P2Rank 0.984
Structure A0A0H3H4R6
Pocket Pocket 1
ColabFold model
FPocket 0.843 · Pocket 3
P2Rank 0.967 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 60 / 4744 genomes with a hit
Normalized 0.013

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0015211 Enables the transfer of a purine nucleoside, a purine base covalently bonded to a ribose or deoxyribose sugar, from one side of a membrane to the other.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0015860 The process in which a purine nucleoside is transported across a membrane. A purine nucleoside is a purine base covalently bonded to a ribose or deoxyribose sugar.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015297 Enables the active transport of a solute across a membrane by a mechanism whereby two or more species are transported in opposite directions in a tightly coupled process not directly linked to a form of energy other than chemiosmotic energy. The reaction is: solute A(out) + solute B(in) = solute A(in) + solute B(out).

Sequence Features

Domain/signature hits from InterPro and related databases.

49 records
Show feature table
Start End DB Term Name
330 340 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
13 394 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
13 394 InterPro IPR036259 MFS transporter superfamily
367 389 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
341 363 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
240 253 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
25 344 Pfam PF07690 Major Facilitator Superfamily
25 344 InterPro IPR011701 Major facilitator superfamily
86 105 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
80 85 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
390 397 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
177 196 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
341 363 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
20 395 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
20 395 InterPro IPR020846 Major facilitator superfamily domain
369 389 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
177 196 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
307 329 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
106 110 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
283 302 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
306 328 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
197 216 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
17 388 PANTHER PTHR43124 PURINE EFFLUX PUMP PBUE
277 282 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
60 79 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
20 42 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
22 388 CDD cd17324 MFS_NepI_like
283 301 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
172 176 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
217 239 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
57 79 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
254 276 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
254 276 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
145 167 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
8 390 SUPERFAMILY SSF103473 MFS general substrate transporter
8 390 InterPro IPR036259 MFS transporter superfamily
86 105 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
364 368 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
217 239 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
111 133 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
110 132 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
145 171 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
21 40 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
41 59 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
302 306 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 394 Hamap MF_01189 Purine ribonucleoside efflux pump NepI [nepI].
1 394 InterPro IPR023680 Purine ribonucleoside efflux pump, NepI
134 144 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3H4R6
AlphaFold — — full sequence — Viewing
ColabFold KP13_00090
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
24 0.716
25 0.517
23 0.319
1 0.235

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 51.16 0.981
2 2.49 0.069
3 1.66 0.028
4 1.58 0.025
5 1.57 0.024