Protein profile

KP13_00116

PTS system N-acetylmuramic acid-specific EIIBC component

Genome: KpKP13

Gene: murP AHE42034.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3H0B2
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Amino acids 477
Annotations 7
Features 49
PDB binders 0
Druggability 0.791

Overview

Basic information about this protein and its source genome.

Accession
KP13_00116
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
murP AHE42034.1
Status
annotated
Amino acids
477
Structure source
AlphaFold + ColabFold
EC
2.7.1.192
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090588 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + N-acetylmuramate (out) = protein cysteine + N-acetylmuramate-6-phosphate (in).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
40.756
DEG E-value
2.44e-99
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.35

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.791
Structure A0A0H3H0B2
Pocket Pocket 6
P2Rank 0.94
Structure A0A0H3H0B2
Pocket Pocket 1
ColabFold model
FPocket 0.817 · Pocket 7
P2Rank 0.923 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 58 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090588 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + N-acetylmuramate (out) = protein cysteine + N-acetylmuramate-6-phosphate (in).

Sequence Features

Domain/signature hits from InterPro and related databases.

49 records
Show feature table
Start End DB Term Name
359 369 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
470 477 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
139 157 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
181 186 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
397 423 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
4 84 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
4 84 InterPro IPR036878 Glucose permease domain IIB
327 337 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
257 281 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
391 396 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
11 43 Pfam PF00367 phosphotransferase system, EIIB
11 43 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
373 390 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
226 245 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
6 89 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
6 89 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
397 419 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
443 469 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 300 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
8 80 SUPERFAMILY SSF55604 Glucose permease domain IIB
8 80 InterPro IPR036878 Glucose permease domain IIB
4 82 FunFam G3DSA:3.30.1360.60:FF:000001 PTS system glucose-specific IIBC component PtsG
117 477 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
117 477 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
5 471 PANTHER PTHR30175 PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN
187 206 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
122 404 Pfam PF02378 Phosphotransferase system, EIIC
122 404 InterPro IPR003352 Phosphotransferase system, EIIC
21 38 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
21 38 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
424 442 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
307 329 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
158 180 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
338 358 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
226 245 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 83 CDD cd00212 PTS_IIB_glc
6 83 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
121 143 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
336 358 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 114 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
370 390 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
207 225 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
265 287 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
448 470 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
158 180 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
115 138 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
187 206 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
301 326 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
246 256 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3H0B2
AlphaFold — — full sequence — Viewing
ColabFold KP13_00116
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
6 0.791
23 0.502
14 0.442

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 26.93 0.918
2 9.54 0.513
3 2.61 0.075
4 2.05 0.045
5 1.36 0.016