Protein profile

KP13_00121

RND family efflux transporter MFP subunit

Genome: KpKP13

Gene: AHE42039.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3H0A8
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Amino acids 374
Annotations 6
Features 22
PDB binders 0
Druggability 0.617

Overview

Basic information about this protein and its source genome.

Accession
KP13_00121
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42039.1
Status
annotated
Amino acids
374
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
50.543
DEG E-value
2.56e-107
Localization
CytoplasmicMembrane
ColabFold pLDDT
87.52

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.617
Structure A0A0H3H0A8
Pocket Pocket 3
P2Rank 0.055
Structure A0A0H3H0A8
Pocket Pocket 1
ColabFold model
FPocket 0.348 · Pocket 3
P2Rank 0.153 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 114 / 4744 genomes with a hit
Normalized 0.024

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records
Show feature table
Start End DB Term Name
8 369 PANTHER PTHR30158 ACRA/E-RELATED COMPONENT OF DRUG EFFLUX TRANSPORTER
16 20 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 20 Phobius SIGNAL_PEPTIDE Signal peptide region
33 364 NCBIfam TIGR01730 efflux RND transporter periplasmic adaptor subunit
33 364 InterPro IPR006143 RND efflux pump, membrane fusion protein
51 273 Gene3D G3DSA:2.40.30.170 -
57 201 Gene3D G3DSA:2.40.50.100 -
47 289 SUPERFAMILY SSF111369 HlyD-like secretion proteins
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
92 165 Gene3D G3DSA:1.10.287.470 Helix hairpin bin
59 282 Pfam PF16576 Barrel-sandwich domain of CusB or HlyD membrane-fusion
59 282 InterPro IPR032317 RND efflux pump, membrane fusion protein, barrel-sandwich domain
273 365 FunFam G3DSA:2.40.420.20:FF:000001 Efflux RND transporter periplasmic adaptor subunit
32 349 Pfam PF00529 Cation efflux system protein CusB domain 1
32 349 InterPro IPR043602 Cation efflux system protein CusB, domain 1
21 374 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 23 SignalP_EUK SignalP-noTM SignalP-noTM
1 18 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
274 366 Gene3D G3DSA:2.40.420.20 -
4 15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 21 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
1 21 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3H0A8
AlphaFold — — full sequence — Viewing
ColabFold KP13_00121
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.408
18 0.006
7 0.004
5 0.0

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 2.48 0.055
2 1.06 0.006