Protein target profile

KP13_00203

putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase

Genome: KpKP13 Gene: AHE42117.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3H4H3
Length 397
Pocket druggability 0.193
Direct ligand evidence 0 84 total records
Functional annotation 1 EC 9 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
55.385 Lower values reduce human off-target concern.
Human E-value
1.78e-149
Gut microbiome similarity
15.0% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Localization

Localization
Cytoplasmic

Structure confidence

ColabFold pLDDT
98.23 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.193
Structure A0A0H3H4H3
Pocket Pocket 2
P2Rank 0.759
Structure A0A0H3H4H3
Pocket Pocket 1
ColabFold model
FPocket 0.174 · Pocket 6
P2Rank 0.629 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 710 / 4744 genomes with a hit
Prevalence 15.0%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

Primary amino-acid sequence viewer.

MRGDFYKQLTSNLETARAEGLFKEERIITSAQQADITVGDSHVINFCANNYLGLANHPELIAAAKSGMDSHGFGMASVRFICGTQDTHKQLEKKLADFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRFRYANNDMQELEARLKEAREAGARHVLIATDGVFSMDGVIANLQGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLEMVEEGADLRDRLWANARLFREKMTAAGFTLAGADHAIIPVMLGEAVVAQNFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQIERAVEAFTRIGKQLGVIA

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:0030170 Binding to pyridoxal 5' phosphate, 3-hydroxy-5-(hydroxymethyl)-2-methyl4-pyridine carboxaldehyde 5' phosphate, the biologically active form of vitamin B6.
  • GO:0009058 A cellular process consisting of the biochemical pathways by which a living organism synthesizes chemical substances. This typically represents the energy-requiring part of metabolism in which simpler substances are transformed into more complex ones.
  • GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0006567 The chemical reactions and pathways resulting in the breakdown of L-threonine.
  • GO:0008890 Catalysis of the reaction: acetyl-CoA + glycine = L-2-amino-3-oxobutanoate + CoA.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0016874 Catalysis of the joining of two molecules, or two groups within a single molecule, using the energy from the hydrolysis of ATP, a similar triphosphate, or a pH gradient.
  • GO:0019518 OBSOLETE. The chemical reactions and pathways resulting in the breakdown of L-threonine (the L-enantiomer of 2-amino-3-hydroxybutyric acid) to form 2-amino-3-oxobutanoate, which is subsequently converted to glycine.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
294 386 FunFam G3DSA:3.90.1150.10:FF:000004 2-amino-3-ketobutyrate coenzyme A ligase
240 249 ProSitePatterns PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site.
240 249 InterPro IPR001917 Aminotransferase, class-II, pyridoxal-phosphate binding site
2 394 Hamap MF_00985 2-amino-3-ketobutyrate coenzyme A ligase [kbl].
2 394 InterPro IPR011282 2-amino-3-ketobutyrate coenzyme A ligase
57 295 FunFam G3DSA:3.40.640.10:FF:000006 5-aminolevulinate synthase, mitochondrial
14 388 PANTHER PTHR13693 CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE
39 387 Gene3D G3DSA:3.90.1150.10 Aspartate Aminotransferase, domain 1
39 387 InterPro IPR015422 Pyridoxal phosphate-dependent transferase, small domain
57 295 Gene3D G3DSA:3.40.640.10 -
57 295 InterPro IPR015421 Pyridoxal phosphate-dependent transferase, major domain
42 386 Pfam PF00155 Aminotransferase class I and II
42 386 InterPro IPR004839 Aminotransferase, class I/classII
5 396 NCBIfam TIGR01822 glycine C-acetyltransferase
5 396 InterPro IPR011282 2-amino-3-ketobutyrate coenzyme A ligase
4 395 SUPERFAMILY SSF53383 PLP-dependent transferases
4 395 InterPro IPR015424 Pyridoxal phosphate-dependent transferase
41 390 CDD cd06454 KBL_like
150 170 Coils Coil Coil

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Site 1 P2Rank #1
0.759
Show in viewer
Surrounding area
Site 2 P2Rank #2
0.174
Show in viewer
Surrounding area
Site 3 P2Rank #3
0.072
Show in viewer
Surrounding area
Site 4 P2Rank #4
0.044
Show in viewer
Surrounding area
Site 5 P2Rank #5
0.021
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Surrounding area
Residue sets
UniProt: Binding site:110-111 in other chain
UniProt: Binding site:135-135
UniProt: Binding site:184-184 in other chain
UniProt: Binding site:209-212 in other chain
UniProt: Binding site:240-243 in other chain
UniProt: Binding site:273-274
UniProt: Binding site:367-367
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3H4H3
AlphaFold DB full sequence Viewing
ColabFold KP13_00203
ColabFold full sequence Loaded

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

84 records
Chemistry signal

Structural and bioactivity evidence are both available for this target.

Direct evidence 0 same-protein records
Transferred evidence 34 records from similar proteins
Structural ligands 33 0 loaded crystals
Measured bioactivity 1 direct and transferred ChEMBL records
Proposed compounds 50 similarity-based ZINC candidates
Best available ligand signal
2BK PDB via homolog 350.3 Da · LogP -0.37 · TPSA 169.4 Open detail RCSB PDB
AKB PDB via homolog Detail RCSB PDB
F9X PDB via homolog Detail RCSB PDB
HR5 PDB via homolog Detail RCSB PDB
J4Q PDB via homolog Detail RCSB PDB

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
2BK RCSB PDB Q0K313 350.3 Da LogP -0.37 TPSA 169.4 1 viol. ✓ Clean Cc1c(c(c(cn1)COP(=O)(O)O)CN[C@@H]([C@H](C)O)C(=…
AKB RCSB PDB P0AB77 117.1 Da LogP -1.01 TPSA 80.4 ✓ Ro5 ✓ Clean CC(=O)[C@@H](C(=O)O)N
F9X RCSB PDB Q0K313 364.3 Da LogP 0.02 TPSA 169.4 1 viol. ✓ Clean CC[C@H]([C@@H](C(=O)O)NCc1c(cnc(c1O)C)COP(=O)(O…
HR5 RCSB PDB P22557 207.3 Da LogP 1.26 TPSA 46.9 ✓ Ro5 ✓ Clean Cc1c(cnn1C)C(=O)NCC2CCC2
J4Q RCSB PDB P22557 243.3 Da LogP 0.79 TPSA 64.8 ✓ Ro5 ✓ Clean CS(=O)(=O)c1nccn1Cc2cscn2
JHP RCSB PDB P22557 227.7 Da LogP 1.75 TPSA 46.9 ✓ Ro5 ✓ Clean Cn1cc(c(n1)C(=O)NC2CCCC2)Cl
JO1 RCSB PDB P22557 221.3 Da LogP 1.41 TPSA 46.9 ✓ Ro5 ✓ Clean Cn1c(ccn1)C(=O)NCc2cccs2
K0V RCSB PDB P22557 242.3 Da LogP 1.38 TPSA 58.6 ✓ Ro5 ✓ Clean CC(C)(C)NC(=O)N1CCC(CC1)C(=O)OC
LUY RCSB PDB P22557 237.3 Da LogP 3.22 TPSA 40.7 ✓ Ro5 ✓ Clean c1ccc(cc1)CCNc2[nH]c3ccccc3n2
NSJ RCSB PDB P22557 209.3 Da LogP 1.94 TPSA 23.5 ✓ Ro5 ✓ Clean c1cc(cc(c1)O)CN2CCSCC2
NSV RCSB PDB P22557 197.3 Da LogP 0.86 TPSA 28.2 ✓ Ro5 ✓ Clean Cc1ncc(s1)CN2CCNCC2
NT7 RCSB PDB P22557 248.3 Da LogP 1.57 TPSA 41.6 ✓ Ro5 ✓ Clean Cc1cccc(c1C)NC(=O)CN2CCOCC2
NTG RCSB PDB P22557 203.2 Da LogP 1.18 TPSA 49.2 ✓ Ro5 ✓ Clean c1cc(ncc1N2CCCOCC2)C#N
NTV RCSB PDB P22557 239.7 Da LogP 2.77 TPSA 38.3 ✓ Ro5 ✓ Clean Cc1c(cccc1Cl)NC(=O)[C@@H]2CCCO2
NU4 RCSB PDB P22557 202.2 Da LogP 2.24 TPSA 55.1 ✓ Ro5 ✓ Clean Cc1c(cco1)C(=O)Nc2ccncc2
NUA RCSB PDB P22557 193.2 Da LogP 1.64 TPSA 46.9 ✓ Ro5 ✓ Clean CCn1cc(cn1)NC(=O)C2CCC2
NUG RCSB PDB P22557 199.3 Da LogP 1.12 TPSA 23.5 ✓ Ro5 ✓ Clean C1CN(CCC1O)CC2=CCSC2
NUJ RCSB PDB P22557 207.3 Da LogP 1.05 TPSA 49.2 ✓ Ro5 ✓ Clean Cc1cc(nc(n1)C)N(C)C2CC(C2)O
NUM RCSB PDB P22557 242.3 Da LogP 1.76 TPSA 62.2 ✓ Ro5 ✓ Clean c1ccnc(c1)C(=O)NCCc2ccc(cc2)O
NUY RCSB PDB P22557 238.4 Da LogP 1.91 TPSA 23.6 ✓ Ro5 ✓ Clean CC(C)N1CCN(CC1)C(=O)c2cccs2
NV7 RCSB PDB P22557 244.3 Da LogP 0.36 TPSA 53.8 ✓ Ro5 ✓ Clean CS(=O)(=O)N1CCN(CC1)Cc2ccco2
NVD RCSB PDB P22557 217.3 Da LogP 1.09 TPSA 46.0 ✓ Ro5 Alert CN(C)c1ccc(cc1)CNn2cnnc2
NVJ RCSB PDB P22557 179.2 Da LogP 1.93 TPSA 38.3 ✓ Ro5 ✓ Clean CCOC(=O)NCc1ccccc1
NVM RCSB PDB P22557 222.3 Da LogP 2.53 TPSA 33.2 ✓ Ro5 ✓ Clean C[C@H]1CCN(C=CC1)C(=O)c2cscn2
NVY RCSB PDB P22557 222.3 Da LogP 2.72 TPSA 15.3 ✓ Ro5 ✓ Clean CCN1CCC(CC1)Nc2ccccc2F
NW4 RCSB PDB P22557 225.3 Da LogP 2.21 TPSA 29.5 ✓ Ro5 ✓ Clean C1CCC(CC1)CCC(=O)N2CCOCC2
NW7 RCSB PDB P22557 235.2 Da LogP 2.09 TPSA 81.2 ✓ Ro5 ✓ Clean CCc1c(c(on1)C)C(=O)Nc2cc(on2)C
PE3 RCSB PDB P09950 634.8 Da LogP -0.81 TPSA 160.5 2 viol. ✓ Clean C(COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO)O
PLG RCSB PDB Q0K313 306.2 Da LogP -0.12 TPSA 149.2 ✓ Ro5 ✓ Clean Cc1c(c(c(cn1)COP(=O)(O)O)CNCC(=O)O)O
PLS RCSB PDB Q93UV0 336.2 Da LogP -0.76 TPSA 169.4 1 viol. ✓ Clean Cc1c(c(c(cn1)COP(=O)(O)O)CN[C@@H](CO)C(=O)O)O
PMP RCSB PDB Q93UV0 248.2 Da LogP 0.16 TPSA 125.9 ✓ Ro5 ✓ Clean Cc1c(c(c(cn1)COP(=O)(O)O)CN)O
SCA RCSB PDB P18079 867.6 Da LogP -1.47 TPSA 400.9 3 viol. ✓ Clean CC(C)(CO[P@@](=O)(O)O[P@](=O)(O)OC[C@@H]1[C@H](…
SIN RCSB PDB P18079 118.1 Da LogP -0.06 TPSA 74.6 ✓ Ro5 ✓ Clean C(CC(=O)O)C(=O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.