Protein profile

KP13_00223

PTS system mannitol-specific EIICBA component

Genome: KpKP13

Gene: mtlA AHE42136.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GUP6
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Amino acids 635
Annotations 8
Features 56
PDB binders 0
Druggability 0.866

Overview

Basic information about this protein and its source genome.

Accession
KP13_00223
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
mtlA AHE42136.1
Status
annotated
Amino acids
635
Structure source
AlphaFold + ColabFold
EC
2.7.1.197
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0022872 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + mannitol(out) = protein histidine + mannitol phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
52.429
DEG E-value
5.79e-171
Localization
CytoplasmicMembrane
ColabFold pLDDT
83.47

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.866
Structure A0A0H3GUP6
Pocket Pocket 29
P2Rank 0.9
Structure A0A0H3GUP6
Pocket Pocket 1
ColabFold model
FPocket 0.544 · Pocket 36
P2Rank 0.92 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 282 / 4744 genomes with a hit
Normalized 0.059

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0022872 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + mannitol(out) = protein histidine + mannitol phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

56 records
Show feature table
Start End DB Term Name
378 465 CDD cd05567 PTS_IIB_mannitol
378 465 InterPro IPR029503 Phosphotransferase system EIIB component, mannitol-specific
99 132 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
490 635 Gene3D G3DSA:3.40.930.10 -
490 635 InterPro IPR016152 Phosphotransferase/anion transporter
377 466 SUPERFAMILY SSF52794 PTS system IIB component-like
377 466 InterPro IPR036095 PTS system IIB component-like superfamily
235 268 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
12 341 ProSiteProfiles PS51104 PTS_EIIC type-2 domain profile.
12 341 InterPro IPR013014 Phosphotransferase system, EIIC component, type 2
161 181 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
378 473 ProSiteProfiles PS51099 PTS_EIIB type-2 domain profile.
378 473 InterPro IPR013011 Phosphotransferase system, EIIB component, type 2
1 19 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
338 358 Coils Coil Coil
366 471 FunFam G3DSA:3.40.50.2300:FF:000047 PTS system mannitol-specific transporter subunit IICBA
335 635 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
8 347 NCBIfam TIGR00851 mannitol-specific PTS transporter subunit IIC
8 347 InterPro IPR004718 Phosphotransferase system, mannitol-specific enzyme IIC
213 234 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
133 154 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
212 234 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
269 292 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
87 109 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
20 42 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
491 635 FunFam G3DSA:3.40.930.10:FF:000007 PTS system mannitol-specific transporter subunit IICBA
293 311 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
52 74 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
495 635 SUPERFAMILY SSF55804 Phoshotransferase/anion transport protein
495 635 InterPro IPR016152 Phosphotransferase/anion transporter
312 334 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
78 98 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
17 277 Pfam PF02378 Phosphotransferase system, EIIC
17 277 InterPro IPR003352 Phosphotransferase system, EIIC
20 42 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
182 212 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
270 292 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
312 334 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
129 151 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
366 471 Gene3D G3DSA:3.40.50.2300 -
498 635 Pfam PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
498 635 InterPro IPR002178 PTS EIIA type-2 domain
539 555 ProSitePatterns PS00372 PTS EIIA domains phosphorylation site signature 2.
539 555 InterPro IPR002178 PTS EIIA type-2 domain
379 453 Pfam PF02302 PTS system, Lactose/Cellobiose specific IIB subunit
379 453 InterPro IPR003501 Phosphotransferase system, EIIB component, type 2/3
494 634 PANTHER PTHR30181 MANNITOL PERMEASE IIC COMPONENT
155 160 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
244 263 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
494 635 ProSiteProfiles PS51094 PTS_EIIA type-2 domain profile.
494 635 InterPro IPR002178 PTS EIIA type-2 domain
48 66 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
43 47 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
498 633 CDD cd00211 PTS_IIA_fru
498 633 InterPro IPR002178 PTS EIIA type-2 domain
67 77 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GUP6
AlphaFold — — full sequence — Viewing
ColabFold KP13_00223
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
29 0.866
3 0.46

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 16.18 0.772
2 5.28 0.247
3 3.68 0.139
4 3.15 0.107
5 2.47 0.068