Protein profile

KP13_00689

Osmolarity sensor protein envZ

Genome: KpKP13

Gene: envZ AHE42309.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3H416
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Amino acids 451
Annotations 5
Features 43
PDB binders 4
Druggability 0.902

Overview

Basic information about this protein and its source genome.

Accession
KP13_00689
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
envZ AHE42309.1
Status
annotated
Amino acids
451
Structure source
AlphaFold + ColabFold
GO
GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
94.977
DEG E-value
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
81.59

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.902
Structure A0A0H3H416
Pocket Pocket 3
P2Rank 0.78
Structure A0A0H3H416
Pocket Pocket 1
ColabFold model
FPocket 0.708 · Pocket 1
P2Rank 0.787 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 111 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Sequence Features

Domain/signature hits from InterPro and related databases.

43 records
Show feature table
Start End DB Term Name
180 232 ProSiteProfiles PS50885 HAMP domain profile.
180 232 InterPro IPR003660 HAMP domain
178 288 FunFam G3DSA:1.10.287.130:FF:000006 Osmolarity two-component histidine kinase EnvZ
233 293 SMART SM00388 HisKA_10
233 293 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
180 232 SMART SM00304 HAMP_11
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
336 438 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
336 438 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
294 446 FunFam G3DSA:3.30.565.10:FF:000018 Two-component sensor kinase EnvZ
160 179 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
217 291 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
217 291 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
332 440 SMART SM00387 HKATPase_4
332 440 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
240 440 ProSiteProfiles PS50109 Histidine kinase domain profile.
240 440 InterPro IPR005467 Histidine kinase domain
12 39 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
294 442 Gene3D G3DSA:3.30.565.10 -
294 442 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
9 437 PANTHER PTHR44936 SENSOR PROTEIN CREC
180 451 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
40 159 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
400 418 PRINTS PR00344 Bacterial sensor protein C-terminal signature
400 418 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
367 381 PRINTS PR00344 Bacterial sensor protein C-terminal signature
367 381 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
385 395 PRINTS PR00344 Bacterial sensor protein C-terminal signature
385 395 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
1 13 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
178 288 Gene3D G3DSA:1.10.287.130 -
177 226 Pfam PF00672 HAMP domain
177 226 InterPro IPR003660 HAMP domain
337 437 CDD cd16950 HATPase_EnvZ-like
234 289 CDD cd00082 HisKA
234 289 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
184 225 CDD cd06225 HAMP
292 440 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
292 440 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
235 289 Pfam PF00512 His Kinase A (phospho-acceptor) domain
235 289 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
160 179 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3H416
AlphaFold — — full sequence — Viewing
ColabFold KP13_00689
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.902
33 0.84

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 10.48 0.563
2 4.77 0.211
3 1.64 0.027
4 1.44 0.019
5 1.41 0.018

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4KP4
P0AEJ4 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.