Protein profile

KP13_00777

Large-conductance mechanosensitive channel

Genome: KpKP13

Gene: mscL AHE42390.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GWI1
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Amino acids 137
Annotations 4
Features 28
PDB binders 0
Druggability 0.578

Overview

Basic information about this protein and its source genome.

Accession
KP13_00777
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
mscL AHE42390.1
Status
annotated
Amino acids
137
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0034220 A process in which a monoatomic ion is transported across a membrane. Monatomic ions (also called simple ions) are ions consisting of exactly one atom. GO:0008381 Enables the transmembrane transfer of an monoatomic ion by a channel that opens in response to a mechanical stress. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
93.431
DEG E-value
4.25e-89
Localization
CytoplasmicMembrane
ColabFold pLDDT
84.94

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.578
Structure A0A0H3GWI1
Pocket Pocket 5
P2Rank 0.093
Structure A0A0H3GWI1
Pocket Pocket 1
ColabFold model
FPocket 0.704 · Pocket 5
P2Rank 0.03 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 1109 / 4744 genomes with a hit
Normalized 0.234

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0034220 A process in which a monoatomic ion is transported across a membrane. Monatomic ions (also called simple ions) are ions consisting of exactly one atom.
  • GO:0008381 Enables the transmembrane transfer of an monoatomic ion by a channel that opens in response to a mechanical stress.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

28 records
Show feature table
Start End DB Term Name
84 92 PRINTS PR01264 Bacterial mechano-sensitive ion channel signature
84 92 InterPro IPR001185 Large-conductance mechanosensitive channel
93 102 PRINTS PR01264 Bacterial mechano-sensitive ion channel signature
93 102 InterPro IPR001185 Large-conductance mechanosensitive channel
6 20 PRINTS PR01264 Bacterial mechano-sensitive ion channel signature
6 20 InterPro IPR001185 Large-conductance mechanosensitive channel
3 134 FunFam G3DSA:1.10.1200.120:FF:000001 Large-conductance mechanosensitive channel
3 132 Hamap MF_00115 Large-conductance mechanosensitive channel [mscL].
3 132 InterPro IPR001185 Large-conductance mechanosensitive channel
97 137 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
3 131 Pfam PF01741 Large-conductance mechanosensitive channel, MscL
3 131 InterPro IPR037673 Large-conductance mechanosensitive channel/anditomin synthesis protein L
74 96 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 127 SUPERFAMILY SSF81330 Gated mechanosensitive channel
12 127 InterPro IPR036019 Large-conductance mechanosensitive channel MscL
1 20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
13 26 ProSitePatterns PS01327 Large-conductance mechanosensitive channels mscL family signature.
13 26 InterPro IPR019823 Large-conductance mechanosensitive channel, conserved site
21 52 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
53 71 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
72 96 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
20 42 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
3 133 NCBIfam TIGR00220 large conductance mechanosensitive channel protein MscL
3 133 InterPro IPR001185 Large-conductance mechanosensitive channel
3 134 Gene3D G3DSA:1.10.1200.120 -
3 134 InterPro IPR036019 Large-conductance mechanosensitive channel MscL
2 136 PANTHER PTHR30266 MECHANOSENSITIVE CHANNEL MSCL
2 136 InterPro IPR037673 Large-conductance mechanosensitive channel/anditomin synthesis protein L

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GWI1
AlphaFold — — full sequence — Viewing
ColabFold KP13_00777
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.554
4 0.046
7 0.006
8 0.006

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 3.32 0.093